| Background and aimsHepatocellular carcinoma(HCC)is the most common malignancy in the liver and is known for its high recurrence rate.Despite the rapid development of liver surgery in the past 100 years,the surgical techniques have been greatly improved,the equipment has been constantly improved,and the curative effect and prognosis of liver cancer have been improved to some extent,the recurrence rate of liver cancer after surgery is still high,which has become the main cause of death of liver cancer patients.Therefore,the study of methods to reduce postoperative recurrence of liver cancer may provide new vitality for patients with liver cancer.At present,studies have shown that liver regeneration after hepatectomy can promote the growth and recurrence of liver cancer,but the specific mechanism remains to be further explored.In order to study the effect of liver regeneration on liver cancer,we first need to build a suitable animal model.Current research in animal models of liver cancer is more for primary liver cancer model,lack of proper postoperative recurrence of liver cancer animal models,this experiment by cultivating the mice liver cancer cell line,build the mice model of partial liver resection,finally will grow in the liver of mice liver cancer cell suspension,successful build after partial liver resection of liver cancer in mice model.To study the mechanism of liver regeneration on liver cancer.With the continuous development of mass spectrometry and the in-depth study of sphingomyelin in recent years,CER has been found to induce apoptosis of cells,which can lead to the occurrence of apoptosis of liver cancer cells.The use of drugs to reduce the production of ceramide expression can reduce the production of TCR-induced interleukin 2(IL2)and programmed cell death.Exogenous ceramide administration or sphingomyelinase induced endogenous accumulation of ceramides,P-AKT-dependent apoptosis induced by ceramide,whichcold inhibit the growth of liver cancer cells,this study focuses on liver regeneration in the tumor immune to changes in the role of liver cancer,and use the quantitative analysis of the sheath of fat of direction,study the change of liver regeneration long chain amide,explore the pathophysiology of liver regeneration while promoting the growth of liver cancer,new ideas for prevention and treatment of recurrence of liver cancer.Methods1.Establish the mouse liver cancer model after partial hepatectomy,and then study the effect of liver regeneration on the growth of liver cancer.2.Explore the changes of liver cancer tissue proliferation,apoptosis level and T cell immunity in the liver regeneration microenvironment,the changes of T cell immune expression in the regeneration liver tissue,and study the effect of the regeneration liver homogenate on liver cancer cells.3.Explore the mechanism of the changes in the expression level of long-chain ceramide on the growth of liver cancer by quantitative analysis of sphingolipids in liver regenerated tissue through the mass spectrometry.Results1.The model of liver cancer in mice after partial hepatectomy was successfully constructed,and the growth-promoting effect of liver regeneration on liver cancer is verified.2.Down-regulated expression of CD8 positive T cells in liver regeneration microenvironment plays an important role in promoting the growth of liver cancer during liver regeneration.3.Down-regulation of long-chain ceramide expression in liver regeneration tissue plays an important role in the decline of liver cancer apoptosis level in liver regeneration microenvironmentConclusionsIn this study,the partial hepatectomy model of liver cancer was successfully constructed and the mechanism of liver regeneration promoting the growth of liver cancer was studied.We found that the down-regulation of the expression of long-chain ceramide in liver regeneration tissue caused down-regulation of the expression of CD8 positive T cells,which together inhibited the apoptosis process of liver cancer and finally promoted the growth of liver cancer.This study provides a new idea for reducing the growth promoting effect of liver regeneration on postoperative recurrence of liver cancer and reducing postoperative recurrence of liver cancer patients. |
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