GC-MS Analysis Of Heat Damaging Kidney Collaterals Syndrome And Kidney Yin Deficiency Syndrome Of Henoch-Schonlein Purpura Nephritis In Children

Objectives:Study urine metabolites of heat damaging kidney collaterals syndrome and kidney yin-deficiency syndrome of Henoch Schonlein purpura nephritis(HSPN)in children by Gas Chromatography-Mass Spectrometry(GC-MS)technology,and compare material basis of the two syndrome types of HSPN,to search the difference metabolic products between excess and deficiency syndrome of HSPN.Then conducting path analysis of metabolites to seek objective indicators what is likely to be existence in excess and deficiency syndrome of Traditional Chinese medicine in HSPN Methods:The urine metabolites of heat injury kidney collateral syndrome group(21 cases),kidney yin deficiency syndrome group(19 cases)and healthy children group(30 cases)of children with Henoch-Schonlein purpura nephritis(HSPN)were measured by Gas Chromatography-triple quadrupole Mass Spectrometer(GC-MS).The relative peak area of the compounds was obtained by XCMS online data processing.The multivariate statistical analysis of the sample data was carried out by using principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)for multidimensional statistical analysis of sample data,selected groups of potential biomarkers,using MetaboAnalyst comprehensive assessment of analysis of related metabolic pathways may be involved in the material basis of purpura nephritis syndrome type children's interpretation and the possible mechanisms.Results:1.The two disease groups of HSPN group and normal group can be distinguished in OPLS-DA,including the OPLS-DA model parameters of R2Y=0.802,Q2=0.205.2.The thermal injury of renal purpura nephritis syndrome group and the normal group can be clearly distinguished in OPLS-DA,the model parameters for R2Y=0.922,Q2=0.342,according to the VIP value is greater than 1,selected 14 kinds of endogenous compounds were a-hydroxyisobutyric acid,Glycolic acid,Propanic acid,Cystathionine,L-Threitol,L-Alanine,Meso-erythritol,Glutaric acid,Citric acid,3-(3-Hydroxyphenyl)-3-Hydroxypropionic acid,D-Mannitol,D-Glucose,N-a-Acetyllysine,Scyllitol.3.The kidney yin deficiency syndrome group and normal group of HSPN were distinguished clearly in OPLS-DA,the model parameters are R2Y=0.985,Q2=0.553,Finally,selected 9 kinds of endogenous metabolites including:Creatinine,1-Aminocyclo-propanecarboxylic acid,Taurine,Palmitic acid,Gluconic acid,Hippuric acid,Stearic acid,Scyllitol,Cellobiose.4.The thermal injury of renal purpura nephritis and kidney yin-deficiency syndrome in the two groups in OPLS-DA can significantly distinguish model parameters are R2Y=0.972,Q2=0.566.Eventually,identified 4 kinds of endogenous compounds:Citric acid,6-Azathymine,N-a-Acetyllysine,Cellobiose.Discussion:1.Compared with the normal group,Taurine decreased in kidney yin deficiency group;Cystathionine were up-regulated in heat injury of renal collaterals group;Lysine decreased in heat injury of renal collaterals group;Scyllo inositol were up-regulated in both heat renal injury group and kidney yin deficiency syndrome group;Citric acid decreased in heat injury of renal collaterals group.2.Compared with the heat injury kidney collateral syndrome group,Lysine,Citric acid,6-Azathymine,Cellobiose showed upward trend in kidney-yin deficiency syndrome.3.All of the metabolites of metabolic pathway analysis,found that the metabolic pathway in children with HSPN mainly involves:Nitrogen metabolism,Cysteine and methionine metabolism,Taurine and hypotaurine metabolism,Pentose phosphate pathway,Citrate cycle(TCA cycle),Lysine degradation.All above results showed that urine metabolites of HSPN and normal group can be clearly distinguished,thermal injury of renal collaterals and kidney yin deficiency syndrome has the difference between compound material basis,mainly related to amino acid metabolism and energy metabolism.