Study Of The Relationship Between CatSper3?SEPT7 And Idiopathic Asthenozoospermia

The spermatozoon is a marvelous cell desigened by nature for the single task of finding an oocyte and initiating the formation of a new embryo.Like many of the most specialized and sophisticated organs and cells of humans,the sperm is fitted with unique components to complete its biosynthetic equipment.As a consequence,for mice,humans,and other mammals,the male stores sperm in a quiescent state.Upon release into the female reproductive tract,the ejaculated �awaken� to begin the obligatory maturational sequence,called �capacitation�,that is required to complete their vital mission-a new embryo.For the past few years,with the deepening of the andrology research and development,checking the sperm morphology and function is becoming more and more popular.At the same time,the quality and function of semen become more and more concerned.Male infertility is closely related to male semen quality,and semen quality may be caused by male infertility.Sperm motility is one of the factors that lead to the decrease of male semen quality.Low sperm motility is called asthenozoospermia(AZS).WHO(World Health Organization,WHO)in 2010 will be given the standard,the sperm motility is divided into three levels: forward movement of sperm(Progressive Motility PR),refers to the sperm fast or slow forward movement;non forward movement of sperm(Non-Progressive Motility NP),refers to the place of sperm movement;sperm activity(Immotility IM).Sperm density?15�106/m L,Progressive motility?32%,is normal sperm motility.Sperm density ?15�106/m L,Progressive motility<32% is Asthenozoospermia.Male semen quality decline is in some of the reasons for male infertility,such as chromosome abnormality,infection,semen liquefaction,varicocele,etc.Unexplained asthenozoospermia is called idiopathic asthenozoospermia.Male infertility caused by asthenozoospermia has become one of the most important subjects in male science.The research on the pathogenesis and the treatment of asthenozoospermia is a difficult problem.There are many factors related to family currently weak sperm,these factor protein molecular pathways and these factors in the occurrence and development of asthenozoospermia etiology to reveal weak sperm,provide clinical treatment basis.This experiment using reverse transcriptase polymerase chain reaction(Reverse Transcription-PCR RT-PCR)and Western blot(Western Blot)were used to detect the expression of normal sperm and asthenozoospermia in CatSper3,SEPT7.CatSper3,SEPT7 and the expression of asthenozoospermia,provide more evidence for the study of asthenozoospermia.Materials and method 1 Objects Thirty asthenozoospermia patients were recruited at The Department of Andrology at the Third Affiliated Hospital of Zhengzhou University in China.Thirty donors were recruited during the period January 2015 through October 2015 at the Human Sperm Bank of the Third Affiliated Hospital of Zhengzhou University.Asthenozoospermia is defined on the basis of progressive(a+b)sperm motility <32%;the fifth percentile of reference values according to WHO(WHO,2010)criteria.The two groups of patients were comparable in age,sexual abstinence,volume,PH,and sperm concentration but differed in progressive motility.2 Methods 2.1 RT-PCR The expression of CatSper3 and SEPT7 mRNA in asthenozoospermia and normal male spermatozoa(control group)was detected by RT-PCR.2.2 Western blottig tests The expression of CatSper3 and SEPT7 proteins in asthenozoospermia and normal male spermatozoa(control group)was detected by Western blot.3 Statistics analysis Data statistics and analysis were using SPSS 22.0 software.The data in the measurement was described by (?)�s.The basic parameters,mRNA and protein expression of semen in the normal control group and the asthenozoospermia sperm group were analyzed by two independent samples t test.Pearson Correlation Analysis supported the correlation sperm motility with the expression of CatSper3 and SEPT7.Statistically significant differences were determined at P<0.05.Results 1 CatSper3 mRNA relative expression level in the two groups RT-PCR detection of the normal control group(normozoospermia),and the asthenozoospermia sperm group showed that the CatSper3 mRNA relative expression level was 1.04�0.33 and 0.55�0.17,respectively,and that CatSper3 mRNA expression was significantly lower in the asthenozoospermia sperm group(t=7.321,P<0.01).2 CatSper3 protein expression level in the two groups Western blot detection in the normal control group(normozoospermia)and the asthenozoospermia sperm group demonstrated that the CatSper3 protein expression level was 0.81�0.08 and 0.41�0.11,respectively,and that CatSper3 protein expression was significantly lower in the asthenozoospermia sperm group(t=15.298,P<0.01).3 SEPT7 mRNA relative expression level in the two groups RT-PCR detection of the normal control group(normozoospermia),and the asthenozoospermia sperm group showed that the SEPT7 mRNA relative expression level was 0.81�0.08 and 0.52�0.06,respectively,and that SEPT7 mRNA expression was significantly lower in the asthenozoospermia sperm group(t=14.930,P<0.01).4 SEPT7 protein expression level in the two groups Western blot detection in the normal control group(normozoospermia)and the asthenozoospermia sperm group demonstrated that the SEPT7 protein expression level was 0.79�0.09 and 0.47�0.11,respectively,and that SEPT7 protein expression was significantly lower in the asthenozoospermia sperm group(t=11.840,P<0.01).5 Correlation between the expression of CatSper3 and SEPT7 and the forward movement of sperm in the two groups Sperm motility and CatSper3 expression levels(r=0.620,P<0.01;r=0.830,P<0.01)and SEPT7 expression levels(r=0.806,P<0.01;r=0.776,P<0.01)were correlated.Conclusions As one of the family menmbers of the sperm calcium channel protein,the expression of CatSper3 in the sperm may lead to the imbalance of calcium channel,the sperm can not be obtained energy,leading to low motility.As one of the family,SEPT7 is an important component of the sperm ring,and the decrease in sperm expression may lead to the loss of sperm structure and low motility.