The Research On The Relationship Between Serum Interleukin-17、Interleukin-33 And Chronic Cough

Objective:Through measuring the serum IL-17 and IL-33 levels in the peripheral blood of patients with chronic cough,to study the relationship between the IL-17 and IL-33 levels and lung function or Fractional exhaled Nitric Oxide in the patients with chronic cough and to evaluate the role of IL-17 and IL-33 in the pathogenesis of chronic cough.Materials and methods:160 patients diagnosed with chronic cough for more than 8 weeks were chosen in the respiratory outpatient department of The First Hospital of Jilin University from Jan 2016 to Jan 2017.The diagnosis of chronic cough in all patients met the 2015 guideline for diagnosis and treatment of cough.The only symptoms of cough or the main symptoms,time more than 8 weeks,chest imaging examination is not obvious abnormalities are known as chronic cough.These patients were first visit,they are 17-70 years of age,gender is not limited;They have good compliance and can cooperate with the relevant examination and follow-up.All patients with the following diseases were excluded:(1)patients with smoking history;(2)patients with systemic or inhaled corticosteroids over the past 4 weeks;(3)patients can not completethe determination of Fe NO and pulmonary function test;(4)patients with mental illness,taking ACEI drugs;(5)patients with heart and cerebrovascular accident or major operation or severity trauma etc nearly six months;(6)patients with alcohol,coffee drinks,high nitrate foods,strenuous exercise on the day of inspection.In addition,60 healthy people were selected who physical check in our hospital as the healthy control group.All selected objects were selected to detect serum IL-17 and IL-33,and all patients with chronic cough were examined by pulmonary function test,Fe NO test and comprehensive allergen test.All data were analyzed by Graph Pad Prism statistical software.The IL-17 and IL-33 levels were compared among chronic cough patients and healthy controls.The correlation between the serum IL-17 or IL-33 levels and lung function or Fe NO levels was further analyzed.Results:(1)we were selected into the group of patients with chronic cough in 160 cases,including 70 cases of male,female 90 cases,age(42.83 ± 5.48)years;healthy controls in the group of 60 people,of which 20 cases of male,female 40 cases,age(43.00 ± 5.30)years;among the groups in age and sex no statistically significant difference(P>0.05).(2)The serum IL-17(64.01 ± 4.70)in patients with chronic cough was higher than that in healthy control group(28.14 ± 2.34),the difference was statistically significant(P<0.05);The serum IL-33(58.06 ± 6.02)in patients with chronic cough was higher than that in healthy control group(31.86 ± 2.66),the difference was statistically significant(P < 0.05).(3)The serum IL-17 level in patients with chronic cough showed a significant negative correlation with the FEV1% of patients(r=-0.6245,P<0.0001);The serum IL-33 level in patients with chronic cough also showed a negative correlation with the FEV1% of patients(r=-0.6722,P<0.0001).(4)The serum IL-33 level of the patients with Fractional exhaled Nitric Oxide concentration(Fe NO)more than 50 ppb was higher than that of Fractional exhaled Nitric Oxide(Fe NO)concentration in 25-50 ppb,and The serum IL-33 level of the patients with Fractional exhaled Nitric Oxide(Fe NO)concentration in 25-50 ppb was higher than that of Fractional exhaled Nitric Oxide concentration less than 25ppb(P <0.05);The serum IL-17 level in Fe NO in <25ppb;25-50ppb;>50ppb;was no significant difference between the three grades,each P between the two groups were more than 0.05.(5)The level of serum IL-33 in patients with chronic cough was positively correlated with the concentration of Fe NO,and the higher the concentration of Fe NO,the higher the level of serum IL-33(rs=0.758,P<0.05).(6)The Fe NO geometric mean of total Ig E<100IU/ml(33ppb)was significantly lower than that of total Ig E>200IU/ml(78ppb)group and Ig E100IU/ml-200IU/ml(69ppb)groups in chronic cough patients(P<0.001).Comparing the last two groups,the P value was found to be0.0824.Conclusion:(1)The levels of serum IL-17 and IL-33 may play an important role in the pathogenesis of chronic cough as an proinflammatory factor.(2)The changes of serum level of IL-17 or IL-33 and the lung function showed a correlation in the patients with chronic cough.(3)The level of serum IL-33 may be involved in the formation of eosinophilic airway inflammation and reflect the severity of eosinophilic airway inflammation.