| 1 Objective Establishment of SD rats Precancerous Breast Lesion(PBL)animal model using carcinogen 7,12-Dimethylbenzanthracene(DMBA).To observe the effect of Xiao He Granule(XHG)on PBL and explore the mechanism of XHG in preventing and treating PBL.2 Methods60 female SD rats without fertility were selected,and 48 rats were treated with DMBA(10mg/100 g body weight),than nine weeks of high fat diet fed rats to establish PBL animal model.Normal Control group(NC group)with 12 rats were fed the same amount of sesame oil.The 48 model rats were randomly divided into four groups——Model group(MC group),Tamoxifen Control group(TC group),XHLC group and XHHC group,and there are 12 rats in each group.Each group was treated for 45 days according to the prescribed dose of drug since about 10 weeks.Using the method of Pathology,IHC and DIPS to observe the general situation of the rats,the amount of food intake,weight change,the appearance of the tumor,pathological changes in breast tissues and detection of Flk-1 and FGF-2 expression in breast tissues.Use statistical knowledge to analyze experimental results and draw conclusions finally.3 Results3.1 Effects of XHG on the general situation of PBL model rats The model of PBL rats' food intake and mood in general aspects have been changed,but due to the limited number of samples,there is no significant difference with NC group in data analysis(P>0.05).The rats in group NC showed no tumor,rats in the MC group the number of visible tumor and the probability of visible tumors was significantly higher than that in XHHC group,the difference was statistically significant(P<0.05),but compared with XHLC group and TC group had no significant difference(P>0.05).3.2 Effects of XHG on pathological morphology of PBL model rats Under the optical microscope to observe the pathological morphology of rat mammary tissue found in rats by DMBA after modeling,performance in group MC showed severe atypical ductal hyperplasia(ADH).The pathological characteristics,using DMBA can establish PBL rat models better.However,TC group,XHLC group and XHHC group were characterized by usual ductal hyperplasis(UDH)and mild ADH pathological features,compared with the MC group,there was significant difference(P<0.01),indicating that XHG and TAM can inhibit and reverse the occurrence and development of PBL.And there was no significant difference between the XHLC group and the XHHC group and the TC group(P>0.05).The results showed that XHG had a significant effect on the treatment of PBL,and the therapeutic effect was similar to that of TAM.3.3 Effects of XHG on Flk-1 and FGF-2 in PBL rats Immunohistochemical method was used to detect the expression of Flk-1 and FGF-2.The expression of Flk-1 and FGF-2 in MC group was higher than that in group NC(P<0.01).XHLC group and XHHC group can reduce the positive area of Flk-1 and FGF-2,can reduce the average optical density(OD)of Flk-1 and FGF-2,compared with the MC group,the difference was statistically significant(P<0.05).And there was a certain dose effect relationship,the effect of XHHC group was better than that of XHLC group,and there was no significant difference between TC group(P>0.05);These results indicate that XHG has a good effect on reducing the expression of Flk-1 and FGF-2 in the mammary tissue of PBL model rats,and it may be the main way to inhibit angiogenesis and reverse PBL.4 Conclusion4.1 XHG can improve the appetite of rats with PBL model,increase the amount of food intake and control the emergence of palpable tumors and the trend,so XHG has therapeutic effect on PBL rats.4.2 XHG can change the morphology of PBL rats mammary gland hyperplasia,which can inhibit and reverse the occurrence and development of PBL in rats.It has therapeutic effect on PBL,and its therapeutic effect on PBL is similar to that of TAM.4.3 XHG has the function of blocking and reversing PBL in rats and the possible mechanism is to inhibit angiogenesis by regulating the expression of Flk-1 and FGF-2,may be the main pathway of XHG to inhibit angiogenesis. |
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