Preparation And Quality Evaluation Of Bisabolol Nanoemulsion

According to the anti-inflammatory activity of bisabolol and advantages of nanoemulsion drug delivery system,the aim of this study was to develop the bisabolol nanoemulsion.To determin the best prescription approach,establish the method of quality evaluation,increase the retention quantity of skin and improve the transdermal delivery ability effectively.Specific research contents include:1.To Determine the content by HPLC:Determined the content determination in vitro of bisabolol nanoemulsion by HPLC.Established the standard curve of bisabolol,methodology study shows HPLC specificity,precision,recovery,stability and repeatability are accord with a standard.HPLC can be used for the content determination in vitro of bisabolol nanoemulsion.2.Study on the Preparation Process:According to the compatibility of the different oil phase and surfactant,using the method of water titration to draw the pseudo-ternary phase diagram of blank nanoemulsion,it is confirmed that the glyceryl triacetate is used as the oil phase,and Tween80 and EL40 are used as the surfactants,the optimum cosurfactant is 1,2-propanediol and Km=1:1.After optimization of prescription,obtained prescriptions for:w(bisabolol)=1.00%,w(bisabolol)=1%,w(glyceryl triacetate)=7.92%,w(EL40)=9.90%,w(Tween80)=4.95%,w(1,2-propanediol)=14.85%,w(H2O)=61.38%.Bisabolol nanoemulsion of O/W was clear and transparent,which preparing by phase inversion composition method.The prescription reached the experimental design requirements.3.Quality evaluation:The morphology of bisabolol nanoemulsion was observed under a transmission electron microscopy.Emulsion droplet uniform distribution of the circular of rules,laser particle size analyzer to determine the average particle size of 11.88 nm,dispersion coefficient(PDI)is 0.108,particle size distribution within 1?35 nm.Staining and dilution method was used to identify the structure pattern of bisabolol nanoemulsion,the results showed that the structure type was oil-in-water.Bisabolol nanoemulsion containing an average dose of 49.88?g/ml.Zeta potential was(-30.2±0.4)mV,bisabolol nanoemulsion still kept clear and keeping the original content after a high-speed centrifugal and acceleration test.It showed that stability of bisabolol nanoemulsion was good.Temperature test shows that content of bisabolol was not obvious change under the low temperature and room temperature.The content of bisabolol declined slightly under a high temperature.So we should avoid storing in high temperature.The stability of bisabolol nanoemulsion is good,the quality evaluation accord with the requirement of emulsion preparation.4.The transdermal absorption:Investigated the permeation volumes of bisabolol nanoemulsion with bisabolol emulsion for comparison by the Franz-type diffusion cells and in vitro mice abdominal skin.Including the permeation volume and transdermal retention volume.The permeation volumes of these 4 different drugs to compare as:Bisabolol Nanoemulsion>Bisabolol Nanoemulsion with 2%azone>Bisabolol Emulsionwith 2%azone>Bisabolol Emulsion.The transdermal retention volumes were 8.74?g·cm-2(Bisabolol Nanoemulsion),3.35?g·cm-2(Bisabolol Nanoemulsion with 2%azone),1.32?g·cm-2(Bisabolol Emulsionwith 2%azone),1.25?g·cm-2(Bisabolol Emulsion)respectively.Both the permeation volumes and transdermal retention volumes of Bisabolol Nanoemulsion were higher than Bisabolol Emulsion observably.Azone has no significant influent on the permeation volumes and transdermal retention volumes of Bisabolol Nanoemulsion.So keeping the transdermal absorption of Bisabolol Nanoemulsion,improving the transdermal retention volumes of Bisabolol Nanoemulsion at the same time.