Expressions Of Lgr5 And ?-catenin In Epithelial Ovarian Cancer Are Associated With SKOV3 Cell Proliferation And Migration

Background and objectiveOvarian cancer is one of the three common malignant tumors of female productive organs.Epithelial carcinoma is the most common histological type,accounting for 85%-90% of the ovarian malignant tumors.Its morbidity has just ranked after cervical cancer and endometrial cancer.However,its mortality rate is the highest in gynecological malignancies.Thus,ovarian cancer has become a serious threat to women's healthy and lives.Due to lacking of symptoms and efficient early diagnostic methods,most of the ovarian cancer patients can not be diagnosed until they are at the advanced stage.Because of high recurrence rate and chemotherapy resistance,the five-year-survival rate of the patients with ovarian cancer is still below 30%.Thus,the solution of early diagnosis,recurrence and chemotherapy resistance issues are the keys to improve the prognosis of these patients.Wnt signaling pathway is involved in embryonic development,regulates cell proliferation,differentiation,movement and apoptosis which is closely related to the regulation of stem cells and the tumor development.It is a complex system involved in a variety of molecules and mutual influence.To investigate the expression of G protein coupled receptor(Lgr5)and ?-catenin in ovarian cancer and its correlation,the effect of inhibiting the expression of LGR5 on the expression of ?-catenin,the cell proliferation and migration in ovarian cancer SKOV3 cells.MethodsIn this study,30 patients with ovarian cancer were collected from the Third Affiliated Hospital of Zhengzhou University from 2014 to 2015,and the expression of Lgr5 and ?-catenin protein in ovarian cancer tissues were detected by immunohistochemistry.The SKOV3 cells were treated by specialized small interference RNAs(shRNA)of LGR5 were transfected into SKOV3 cells.The expression levels of Lgr5 and ?-catenin proteins were detected by Western Blot.The relative expression levels of LGR5 mRNA and?-catenin mRNA were detected by qRT-PCR.The level of cell proliferation was detected by CCK-8 and the cell migration was detected by scratch assay.SPSS19.0 statistical software was used to analyze database and the correlation analysis.Count data was analyzed by Pearson's Chi-square test or Fisher Exact test and the correlation between two rank variables was analyzed by Spearman rank correlation analysis.All the tests were two-sided with ?=0.05 as the inspection level.P<0.05 is considered to be statistically significant difference.ResultsThe espression of Lgr5 and ?-catenin in ovarian cancer had significant difference compared with normal ovarian tissue or with ovarian serous cystadenoma(P<0.05).There was positive correlation between the expression of Lgr5 and?-catenin in ovarian cancer(r=0.40,P<0.05).After inhibiting the expression of LGR5 in SKOV3 cells,the relative expression levels of?-catenin mRNA and the expression levels of?-catenin protein were significantly lower than the other control groups(P<0.05).The inhibitory rate of cell proliferantion and cell migration had significant difference compared with the other control groups(P<0.05).Conclusion1 Lgr5 and ?-catenin are both over expressed in ovarian cancer tissues.It indicates that both of them may play a synergistic role in the development and progression of epithelial ovarian cancer.2 The expression of Lgr5 play an important role in SKOV3.It indicates that Lgr5 maybe regulate the SKOV3 by?-catenin.3 Lgr5 maybe promote the proliferation and migration of SKOV3 cells.It indicates that Lgr5 may play an important role in the development,invasion and metastasis of epithelial ovarian cancer.