Study On Pharmacology Barriers On Cerebral Ischemic Injury An Behavior Associated With Tibetan Medicines

The study found that the national drug in the treatment of stroke and related sequelae of clinical treatment is still maintained a rising trend,of which the most prominent Tibetan Medicine.In the classic Tibetan medical records,the combination of the three methods with blood,lung veins,and long are all used on the stroke of disease for specimens purpose.Overall,the Tibetan Medicine experience and unique Chronopharmaceutical drug delivery theory is the treatment of cerebral ischemic diseases.The purpose of this experiment is to investigate the efficacy of the combined using of these three kinds of Tibetan Medicine,and explore the rationality of its dosing method.1.MCAO/R model was established and Nissl staining of brain tissue was used to observe the changes of Nissl bodies.Can effectively alleviate cerebral ischemia damage Nissl body injury caused by combined use of the Tibetan name,maintaining the integrity of neuronal structures.Using ICH/S-P assay and RT-PCR results show that the combination of Tibetan medicine prescription can effectively promote the protein content of Bcl-2,NF-?Bp65,ICAM-1,down-regulation of Caspase-3,VEGF,TLR4 protein expression,inflammatory reaction and apoptosis caused by cerebral ischemic injury has good effect of the intervention.2.The establishment of MCAO/R model,the detection results of blood rheology indicators show that can significantly reduce vascular ischemic stroke within the whole blood viscosity,plasma viscosity in conjunction with the Tibetan side,ESR,Hct,equation K value of ESR,EAI,erythrocyte rigidity index.The electrophoresis index and erythrocyte deformability index were significantly increased,and the mechanical properties of blood flow were improved.With the blood of mice in acute model show that the Auricle Microcirculation detection:Tibetan name combined with a significant expansion of acute blood in model mice induced by adrenalin arteriole and venule diameter of blood vessels,improve blood flow,improve microcirculation.This experiment combined with acute blood MCAO/R model and mice model,to discuss the three kinds of medicine prescription blood stasis,dilation of blood vessels,promote blood flow,improve the protective and therapeutic effects of vascular function.3.To establish a mouse model with acquired memory disorder mice,used increasingly dark habits of learning,memory,reaction ability test results show a significant increase in mice latency associated with Tibetan medicine,the number of test results and active shuttle,better training performance,protect the brain injury induced by M receptor inhibitors in mice,reduce the learning and memory impairment in mice produced symptoms of reaction reduce;AChE,MDA activity in the brain tissue of mice,significantly increased the content of SOD,maintenance of cholinergic nerve function,remove free radicals,reducing lipid peroxidation injury.