Evaluation Of The Antioxidant Activity Of "medicine Food Homology" Plant Extract Water

This paper extracts the active ingredients of Cili,ginger,cinnamon,yam,grape seed as raw materials,using Caenorhabditis elegans,(C.elegans)as a model to evaluate the antioxidant effects by lifespan and oxidative stress assays.The results have a diet guidance on human,and also prove that these plants have anti-aging and antioxidant value.In addition,on the basis of the research of "medicine and food homology" plant,this paper deeply studied a kind of natural plant’s antioxidant effect on C.elegans.The plant’s name is Pine pollen.In this paper,the physiological indexes and the anti-oxidative stress were evaluated by the water extract of pine pollen,the results showed that 2mg/ml pine pollen extract had significant difference on the life span compared with N2.2mg/ml pine pollen have more eggs than control group,and the swallowing rate was generally higher than control.However,the high concentration of pine pollen had little effect on the movement ability of nematodes.In addition,the oxidative stress resistance experiment,1mg/ml aqueous extract of pine pollen have significant thermal resistance protection ability compared with control group.The results showed that the water extract of pine pollen had protective effects on the nematode in different degree.Otherwise,the water extract of pine pollen had a better protective effect on the nematodes induced by 10 mM oxidative stress compared with the control group.The results indicated that the aqueous extract of pine pollen had a good protective effect against oxidative stress induced by hydrogen peroxide.To sum up,C.elegans was using as a model to evaluate the antioxidation effects of medicinal and edible plants and pine pollen.The results provide the experimental basis for medicinal and edible plant on antioxidant and anti-aging effect.