The Repair Effect On Skin Wound And Safety Evaluation Of Nano–Electrospun Composites

The skin will form wounds in the surface when it stimulated by the external stimulus such as trauma,surgery and burns.Skin damage can cause metabolic disorders,water and a variety of nutrients quickly consumed easily and then lead to the immunity decreased.It may cause other complications and severe cases will be life-threatening if not treat timely.Therefore,it is very important to repair the wound as soon as possible after the skin is damaged.Traditional medical dressings are mainly made of absorbent cotton gauze,gauze and cotton and other natural fibrous materials,they can dry wounds,and they have physical isolation function.Although the traditional dressings are simple making and cheaper than others,but the traditional dressings often scab with exudates,they will cause the wound adhesion,while change the dressing the wound adhesion may bring out new trauma as well as pain.At the same time traditional medical dressings are easy to cause bacterial growth,and with poor functions of moisturizing and hemostasis.Therefore,looking for a new type of dressing has become the focus of current research.New dressings should satisfy the following requirements: The new dressings' biocompatibility is good,they cannot stimulate,poisoning,disease or other adverse reactions to the human body;The new dressings with moisturizing and dilution effect can retain the effusion effectively at the same time no fluid;Then the new dressings shouldn't be easy to stick with the wound effusion,they should minimize the damage to the new tissue,when change the dressings the pain is light;Finally,the new dressings should have the role of prevention of wound infection.Objective: To study the repair effect and safety evaluation of b FGF-CUR / PLGA nano-electrospinning composites on skin wounds.Methods: In this study,biodegradable polymer polylactic acid-glycolic acid(PLGA)was used as a carrier to support curcumin(CUR)and basic fibroblast growth factor(b FGF),the biodegradable and sustained-released nano-electrospun composites were prepared by electrostatic spinning method.The microstructure of the material was observed by scanning electron microscopy(SEM).The composites were characterized by scanning electron microscopy,infrared spectroscopy and X-ray diffractometer.The composites were covered on the wound surface of the rat model to observe the cure rate at different time.The cell proliferation test,skin acute toxicity test,skin irritation test and skin allergies test were used to evaluate the safety of b FGF-CUR / PLGA nano electrospinning composites.Results:The PLGA nano-electrospun composites with growth factor and curcumin did not change the microstructure,the fiber diameter was smooth at the nanometer level,and the thickness was uniform and interwoven.The results of infrared spectroscopy showed that the three materials were well mixed together and the relative bond was not formed.The drug was encapsulated in the PLGA structure to form the inclusion compound,which increased the stability.X-ray diffraction analysis showed that the b FGF-CUR/PLGA nanofilm composites had higher strength than that of the electrospinning PLGA material,which indicated that the addition of curcumin of small molecular substances improved the crystal structure of the nano-electrospun composites to a certain extent.The drug release test showed that the cumulative release percentage of b FGF-CUR/PLGA nano-electrospun composites with time could be better to maintain the sustained release effect of drug.The general observation of the wound showed that the healing level of curcumin and b FGF group was significantly better than that of the blank control group.The results showed that: The b FGF-CUR/PLGA nano-electrospun composites with different percentage of b FGF(8.33 × 10-4%,16.67 × 10-4% and 33.33 × 10-4%,respectively)all promoted the cells on the surface of the dressing growth and proliferation,there were no skin acute toxicity,skin irritation or skin allergies.Conclusions: In this study,biodegradable polymer PLGA was used as carrier,supported CUR and b FGF.The composites slow release material prepared by electrostatic spinning method had good nanostructure,promoted wound healing,and no acute toxicity,irritation or sensitization,contributed to cell growth and proliferation.The composites could meet the medical application,in the field of drug-controlled release and biodegradable nano-dressings had a potential medical value.