| The differences of the immunity activities of LPG2 and LPX3 on macrophages activation and mesenteric lymph node cells proliferation were investigated. The mechanism of LPG2 on macrophages was measured by the method of adding cell receptor inhibitors and the real time fluorescence quantitative PCR was investigated. The model of mice stimulated by LPS was adapted in order to analyze the effects of raw dry longan polysaccharides on intestinal physiological status, myeloperoxidase, lipocalin-2, immune globulin and IL-6 and IFN-y secretion levels in plasma. The main results were showed as follow:1. Both LPG2 and LPX3 could activate the macrophages and enhance the proliferation of mesenteric lymph node cells. At 400 ?g/mL, compared with control group,the proliferation index of macrophages stimulated by LPG2 and LPX3 were 143.70% and 135.90%, respectively; the phagocytosis levels were 198.28% and 192.03%. The production of TNF-? and IL-6 secreted by macrophages exposed to 400?g/mL LPG2 were 528.65 pg/mL and 109.22 pg/mL, respectively. Compared with control group, the enhancement levels were 31.54 times and 27.31 times, respectively. The TNF-a and IL-6 secretion released by macrophages stimulated by 400 ?g/mL LPX3 were were 296.20 pg/mL and 73.69 pg/mL, respectively. The enhancement levels were 17.67 times and 18.42 times, respectively. The NO production secreted by macrophages exposed to 200 ?g/mL LPG2 and LPX3 was 4.6 times and 4.4 times comparing with the blank group, respectively.At the same time, both LPG2 and LPX3 could improve the proliferation of mesenteric lymph node cells in a dose dependent manner in the range of 6.25?200 ?g/mL. Compared with the same concentration of LPX3, LPG2 displayed stronger effects on proliferation and TNF-? and IL-6 secretion aspects of macrophages.2. LPG2 could activate the macrophages via MyD88-NF-?B pathway mediated by TLR4 and TLR2. The anti-TLR2 and anti-TLR4 could significantly attenuate the phagocytosis and decrease the TNF-a and IL-6 secretion levels of macrophages stimulated by LPG2. Compared with the LPG2 group, the effects of anti-TLR2 and anti-TLR4 on the phagocytosis decrement levels were 67.23% (p<0.05) and 79.46% (p<0.05), repectively.The TNF-a decrement levels were 89.28% (p<0.05) and 89.93% (p<0.05), repectively,which had a significant defference compared with the control group(p<0.05). The IL-6 decrement levels were 73.21% (p<0.05) and 81.21% (p<0.05), which had no significant deffemce compared with the control group (p>0.05). At the same time, the qPCR showed LPG2 could improve the gene expression of TLR2 and TRL4 and TRIF, MyD88 and NF-?B in TLR4 and TLR2 downstream signaling pathways. The results demonstrated that LPG2 could activate the marophages via MyD88-NF-?B pathway mediated by TLR4 and TLR2.3. The raw longan polysaccharides (LP) purified from dry longan pulp could enhance the physiological status of mice stimulated by LPS. Compared with the control group fed common feedstuff for ten weeks, the dry longan polysaacharide could decrease the liver index of mice fed feedstuff with LP (4g/100g) and relieve the condition of liver and intestinal tract inflammation stimulated by LPS. The MPO and LCN-2 levels in mice fed polysaacharide decreased significantly. Compared with the control group, its decrement levels were 47.01% (p<0.05) and 20.38% (p<0.05). While the IL-6 and IFN-? levels increased by 8.79% and 50.29%, respectively. Compared with the control group, IgA, IgM and IgG increased by 37.02% (p<0.05), 40.14% (p<0.05) and 18.26% (p<0.05),respectively. All these results demonstrated that LP could enhance the immunity of mice and improve the physiologic state stimulated by LPS.The results showed that Both LPG2 and LPX3 could activate the macrophages and enhance the proliferation of mesenteric lymph node cells. Compared with LPX3, the activity of LPG2 enhancing the proliferation and cytokines secretion of macrophages was stronger. LPG2 could activate the marophages via MyD88—NF-?B pathway mediated by TLR4 and TLR2. The polysaccharide purified from dry longan pulp could enhance the immunity of mice stimulated by LPS. All these results could pave the way for further development of the longan and special resources in China. |
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