| bjective: Cerebral infarction is defined as cerebral circulation disorder caused by various factors and local brain tissue become hypoxic ischemic necrosis,which result to limb dysfunction,aphasia,ataxia and other clinical manifestations.Drug and surgical treatment can reduce the patients' mortality,save the lives of patients,but the cause for damaged brain sequelae such as cognitive impairment,motor dysfunction,sensory dysfunction,speech dysfunction etc.are very difficult to cure,which seriously affect the activities of daily living of patients,resulting in a decline in the quality of life of patients.The technology of repetitive transcranial magnetic stimulation(rTMS)as a new kind of Non pharmacological treatment of nervous system,with painless,non trauma and other physical characteristics,which can alleviate various central diseases and promote the recovery of patients' function,has been widely used in the treatment of post-stroke Rehabilitation,neuropathic pain,epilepsy,peripheral nerve disease,spinal cord injury and other diseases,has achieved the certain curative results,This experiment aims to study the effects of transcranial magnetic stimulation on the memory function and expression of synaptophysin in hippocampus of rats with cerebral infarction,providing guidance basis of rTMS treatment on the rehabilitation of patients with cerebral infarction for further application.Methods : 75 adult male SD rats,weighing 200~250g,were established with right middle cerebral artery occlusion(MCAO)of cerebral infarction model by suture method.Model rats were randomly divided into a model group and 40%(0.76T)?60%(1.14T)?80%(1.52T)maximum intensity stimulation group(n =15),another in the sham operation group(n = 15).The rats in rTMS group receive transcranial magnetic stimulation treatment at the next day after the infarction for 5 days,the sham operated group and the model group do not accept magnetic stimulation treatment.After this treatment,10 rats selected from each group randomly were given the same foot shock,recording the duration in rats of Stupor response in 5 minutes after 24 h..At the same time detect the expression of synapses in the hippocampus of another 5 rats in each group by immunohistochemistry in 1 d,7 d,14 d,21 d,28 d after the treatment.Results: 1.in fear memory experiment: The duration of stupor response of rats in sham operation group receiving footshock after 24 h were significantly higher than that of stimulation group and model group(p<0.01),and the stiffness duration of stimulation group were higher than that of model group,in the stimulation groups,60%(1.14T)magnetic stimulation group has the longest duration of stupor response.2.Under the fluorescence microscope: The SYN immunoreactivity in the hippocampus of rats was the highest in the sham operation group,uniforming distribution of each time period,whih no obvious correlation between density and action time(p>0.05).There is no obvious difference between model group and stimulation group of SYN positive Things count in the short term,but with the effect of r TMS on the brain central nervous system is prolonged,the two groups in rat hippocampal CA1 region SYN immunoreactive material were significantly increased,and in the stimulation group,60%(1.14T)magnetic stimulation group has more synaptophysin positive cells and largest increase(p<0.05).Conclusion: 1.Repetitive transcranial magnetic stimulation can promote the recovery of memory function in rats with cerebral infarction;2.rTMS has the positive role on the expression of synaptophysin in hippocampus of rats with cerebral infarction.3.Medium intensity stimulation of r TMS has highest recovery of memory function and increased the maximum number of the hippocampus synaptophysin positive cells in rats with cerebral infarction. |
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