A Study On The Relationship Between The Single Nucleotide Polymorphism Of TSPAN8 Gene And Susceptibility Of Hepatocellular Carcinoma Pedigrees In Fusui County Of Guangxi Zhuang Autonomous Region

Objective: The present study aimed to investigate the relationship between functional single nucleotide polymorphisms(SNPs)of TSPAN8(rs1051334 and rs2270587)and susceptibility of hepatocellular carcinoma(HCC)pedigrees in Fusui County of Guangxi Zhuang Autonomous Region.Methods:This case-control study included 20 high incidence HCC families(20HCC cases and 59 immediate family members)and 10 health control families(40 cases).Genotyping was carried out by Matrixassisted laser desorption/ionization time of flight(MALDI-TOF).SPSS software,version 17.0,performed all statistical analysis.A two-tailed P-value of less than 0.05 was considered a statistically significant result.According to the law of Hardy-Weinberg equilibrium,we were test all groups genotype alignment.The difference of genetic frequencies was perpormed?~2 test.Non-conditional logistic regression model was used for all analysis variable to analyze the association of TSPAN8(rs1051334 and rs2270587)SNPswith susceptibility of HCC families.Results:1.(1)The frequencies of the TSPAN8 gene rs1051334 genotypes TT,TG and GG were 55.7%?34.2% and 10.1% respectively among the HCC high incidence families.The frequencies of the TSPAN8 gene rs1051334 genotypes TT,TG and GG were 62.2% ? 35.1% and 2.7% respectively among the control families.There was no significantly difference in genotypes frequencies distribution between two families.(2)The frequencies of the TSPAN8 gene rs2270587 genotypes CC,CT and TT were 67.1% ? 31.6% and 1.3% respectively among the HCC high incidence families.The frequencies of the TSPAN8 gene rs2270587 genotypes CC,CT and TT were 50.0%?45.0% and 5.0% respectively among the control families.HCC high incidence families were not significantly Difference from control families in genotype frequencies distribution.2.(1)The frequencies of the TSPAN8 gene rs1051334 T allele were 72.5%?72.9% and 79.7% and G allele were 27.5% ? 27.1% and 20.3% respectively among the HCC group of HCC families,non-HCC group of HCC families and control group.Alleles' distribution were not statistical significance difference neither HCC group of HCC families and non-HCC group of HCC families(?~2=0.002,P=0.963)nor HCC group of HCC families and control group(?~2=0.771,P=0.380).In the non-HCC group of HCC families,HCC onset risk of individuals with GT and GG genotypes of TSPAN8 were 1.15 fold(95%CI=0.32~4.13)and 0.62 fold(95%CI=0.08~4.87)of individuals with TT genotype respectively.But no significant difference was observed(P>0.05).In the control group,HCC onset risk of individuals with GT and GG genotypes of TSPAN8 were 2.82 fold(95%CI=0.52~15.27)and 3.61fold(95%CI=0.21~63.44)of individuals with TT genotype respectively.But no significant difference was found(P>0.05).(2)The frequencies of the TSPAN8 gene rs2270587 C allele were 90.0%?80.5%and 72.5% and T allele were 10.0%?19.5% and 27.5% respectively among the HCC group of HCC families,non-HCC group of HCC families and control group.Differences among those with alleles distribution were not statistically significant between HCC group of HCC families and non-HCC group of HCC families(?~2=1.899,P=0.168).Differences among those with alleles distribution were statistically significant between HCC group of HCC families and control group(?~2=4.812,P=0.028).In the control group,HCC onset risk of individuals with T allele of TSPAN8 were 0.29 fold(95%CI=0.09~0.92)of individuals with C allele.In the non-HCC group of HCC families,HCC onset risk of individuals with CT genotype of rs2278587 sites were 0.42 fold(95%CI=0.11~1.66)of individuals with CC genotype.But no significant difference was found(P>0.05).The odds ration of HCC for people with TT genotype could not be calculated.In the control group,HCC onset risk of individuals with CT genotype of rs2278587 sites were 0.34 times(95%CI=0.07~ 1.59)of individuals with CC genotype.But no significant difference was observed(P>0.05).The odds ration of HCC for people with TT genotype could not be calculated.Conclusions:1.For the SNPs of TSPAN8 gene rs1051334 and rs2270587,the genotype frequencies in both groups complied with the Hardy-Weinberg equilibrium in Fusui of Guangxi.2.The TSPAN8 gene rs1051334 SNP may not be correlated with susceptibility of HCC high incidence families in Fusui of Guangxi.3.TSPAN8 gene's polymorphism might be related to susceptibility to HCC in Fuisui of Guangxi.Rs2270587 T allele might be a protective factor of HCC.