| Objective: To study the effect of distal vasectomy on the total numbers of various types of cells in the rat testicular interstitial tissue.Methods: 12 male Sprague-Dawley rats were subjected to unilateral distal vasectomy via the inguinal canal.37 days after operation,bilateral testes were removed and methacrylate resin-embedded sections prepared.After the sections were stained with periodic acid-Schiff's reagent and hematoxylin,histological observation was carried out and the total numbers of all types of cells(except erythrocytes)in the interstitial tissue were quantitatively studied using a stereological method,the optical disector.Results: In comparison with the non-vasectomized side,the spermatogenesis in the testis on the vasectomized side was markedly damaged in 5 of the rats.No matter whether there was spermatogenic damage,the total number of the Leydig cells,endothelial cells or myoid cells in the testicular interstitium was not significantly changed on the vasectomized side.Conclusion: Short-term vasal ligation has no effect on the numbers of testicular interstitial cells,with its effect on the spermatogenesis being unrelated to the number of Leydig cells. |
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