| As we all known,China is the country with the world's most population.1.38 billion compatriots give us the demographic dividend but also bring various problems what we have to face.Aging,trauma makes China more than 100 million cartilage injury patients.With the aging process,more and more of such patients are appearing.Because of their characteristics,cartilage can't be effectively repaired after damage,and the accumulation of cartilage damage will eventually lead to the lose of labor capacity.This current situation come up with a huge problem to the society and the medical profession: how should we save the cartilage injury patients.In recent years,the answers to this problem is focusing to the field of tissue engineering,using tissue engineering techniques to repair cartilage damage has been widely agreed and researching.Tissue engineering refers to the make bioactive substances materials of biological material or artificial material in vitro,then implant the material into body replacing the function of organs or tissues.Because a large number of applications of tissue engineering in the reconstruction of the organs and tissues of medicine,tissue engineering is also known as regenerative medicine.On the design of experiment,using chitosan and hydroxyapatite as similar physical and chemical properties to bone cartilage for materials preparation respectively in the cartilage and bone tissue repair.To promote cartilage repair function,Kartogenin active small molecules as the active factor of the cartilage repair would be load,to strengthen the support into cartilage performance.Experimental Method In this experiment,hydroxyapatite is consistent with human bones composition and chitosan is contribute to cartilage repair.The two substances are the main components of the scaffold material,and assisted by microwave rapid synthesis to produce hydroxyapatite nanoparticles with the same size.Then the micro-nano hydroxyapatite particles are analysised by X-ray diffraction,Thermogravimetric and Differential Scanning Calorimetry,specific surface area analysis,transmission electron microscopy and scanning electron microscopy to study particle properties.Because of the hydration layer and high specific surface area of hydroxyapatite,we use hydroxyapatite to load Kartogenin as an active molecule.Test the Kartogenin laden hydroxyapatite chitosan scaffold's physical properties,detect its biological activity,cell toxicity and eventually will implant into animals' osteochondral defect,then observe the repair effect.experimental resultExperimental diameter was 1.158 ± 0.148 um spines of micro-nano hydroxyapatite hollow ball.The hollow ball's specific surface area of 78.17 m2 / g.BJH Stripping pore volume of 0.54 cm3 / g.Average stripping aperture is 1.01 nm,the scaffold's void fraction is from 96.1% to 96.1%,sscaffold aperture is in 50-120 um,can slowly release Kartogenin and stable release calcium phosphate ions.Thescaffold also showed good biocompatibility.Bone marrow mesenchymal stem cells are implantedontoscaffold,then after 3 days can be found the cellson the scaffold with good adhesion,spreading and breeding behavior.Animal experimental results show that the load KGN hydroxyapatite scaffold can provide certain help for osteochondral defect repair.Conclusion : The experimental results show that the load KGN hollow micro-nanometer hydroxyapatite spheres chitosanscaffold has good physical and chemical properties and biological properties,with further research prospects in bone cartilage repair. |
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