Study Of Biocompatibility Of Nano-Fluoridated Hydroxyapatite/Poly (D,L-lactide) Composite Scaffold

Hydroxyapatite (Ca10(PO4)6(OH)2,HA) was widely used for bone tissue engineering due to its good bioactivity and biocompatibility. Nano-HA/PDLLA composite scaffold can simulate the cellular matrix environment and promote cell activity and proliferation. One of the characteristics of hydroxyapatite is that the hydroxy group of hydroxyapatite can be substituted by fluorine. It has been reported that fluoridated hydroxyapatite (Ca10(PO4)6Fx(OH)2-x, FHA) could promote cell proliferation. These results suggest that FHA a promising biomaterial.In this thesis, a number of nano-FHA with different F content were fabricated. The nano-FxHA/PDLLA porous composite scaffolds were also prepared using solvent casting/particulate leaching method. These materials are potentially ideal candidates for bone tissue engineering scaffold or bone defect repair due to the well-defined biodegradation characteristics of PDLLA and biocompatibility of FHA nanocrystals. This work first investigated the influence of different F content in FHA/PDLLA on the behavior of the cells cultured. It was found that the F content in these newly manufactured materials was suitable for cell proliferation and differentiation. In vivo experiments indicated the feasibility of the FxHA/PDLLA with the suitable F content for bone tissue engineering scaffold or bone repair materials.In vitro cell culture was used to evaluate the cytocompatibility of FxHA/PDLLA(x=0.25, 0.5, 0.75, 1.0, 1.6) and examoine the influence of F content of FxHA/PDLLA on cell proliferation and differentiation. The results of in vitro experiments showed that F0.25HA/PDLLA could promote cell proliferation and differentiation, and had a good cytocompatibility. In vivo experiments results also suggested that F0.25HA/PDLLA had an obviously bone repair ability. Therefore, the nano-F0.25HA/PDLLA could be used as a new bone tissue engineering scaffold or bone repair material.