Natural Aging And Damage Resistance Of Basal Forebrain Nestinpositive And Nestin-negitive Cholinergic Neurons

Background:Basal Forebrain is a highly heterogeneous region with a varity of nerons linked to different brain regions and there is no obvious boundary to other brain regions.Basal forebrain neurons containing many different chemical neurons mainly include cholinergic neurons,GABAergic,glutamatergic and other neurons.Recent studies provided that there is a group of mature nestin-immunoreactive(nestin-ir)neurons in the basal forebrain of adult rats and human.In recent years,nestin-ir neurons have been found to play an important role in the learning and memory of the brain and the damage and repair of the central nervous system.Compared with Nestin negitive cholinergic neurons,nestin-ir neurons have stronger anti-injury ability and neural plasticit.ObjectiveThis research used cellular immunofluorescence to study the different of nestinir neurons and nestin negitive neurons on natural aging,and the ability of the two neurons on repairing after oxidative damage.In order to understand the neural plasticity of the two neurons.In order to provide new ideas to the nervous system diseases.MethodsThis experiment uses about eight neonatal SD rats(<24h)each time.Neurons in basal forebrain are cultured in culture plate for 1 day,3 days,5 days,7 days,Day,9days and 11 days,we use the basal forebrain neurons to do immunofluorescence experiment on each time point.Anti-injury study: Using about eightneonatal SD rats,and neurons in the basal forebrain were cultured in culture plate for 3 days,then added150?mol / L H2O2 into plate for construct a cell injury model.After 12 h,the basal forebrain neurons were used to do immunofluorescence experiment.Then we use fluorescence microscopy to observe the difference resistance to oxidative damage of the two neurons.ResultsThe growth of cells reached the peak on the third and fifth day,in this time neurons are larger,and neuronism are formed.Some synapses net are formed between some neurons.Among them,nestin-ir neurons are larger,and have large and long protrusions,although nestin negative neurons are small,and have less protrusive.The number of neurons decreased gradually after five days,the cell body saturation decreased,the number of nestin-ir cholinergic neurons decreased gradually,the proportion of total cells increased gradually,the number of nestin-negative neurons decreased rapidly,and the proportion of the total number cells decreased rapidly.Nestin-ir cholinergic neurons and negative cholinergic neurons under the same condition,the degree of natural aging is different.Nestin-ir cholinergic neurons decreased slowly than nestin negative cholinergic neurons.In the peak period of neuronal growth,nestin-ir cholinergic neurons survived more viable cells and had better cell morphology and strong anti-injury ability than nestin-negative cholinergic neurons in the experiments.Using the third day neurons in the anti-oxidative damage experiments,nestin-ir cholinergic neurons showed a stronger anti-injury ability.ConclusionsThe nestin-expressing neural cells(NECs),which are positive in the basal forebrain.They have better physiological activity than the nestin negative neurons,and nestin-ir cholinergic neurons have stronger excitability and better resistance aging ability of neural plasticity.Nestin-ir cholinergic neurons show greater neural plasticity after oxidative damage.Nestin-ir cholinergic neurons are active in the functional state,so that the basal forebrain has specific functions,such as anti-injury function and loss of repair function.