The Impact Of Isoliquiritigenin On Biological Behaviors Of Human Gastric Carcinoma Sgc7901 Cells And Its Possible Mechanisms

Objective: To investigate the effect of isoliquiritigenin on biological behaviour of human gastric carcinoma SGC7901 cells and its molecular mechanisms, provide reliable theoretical basis for future clinical application.Methods : The logarithmic phase human gastric carcinoma SGC7901 cells were divided into control group(normal cell culture fluid) and isoliquiritigenin group(isoliquiritigenin soluble in cell culture fluid, the concentration respectively was 10?25?50 and 100 ?mol/L), each group had four repeat holes. The proliferation of SGC7901 cells were detected with MTT assay after 24 h, 48 h and 72 h of culture,groped for subsequent experimental drug concentration and action time; The in vitro invasion abilities of SGC7901 cells was assessed with Transwell test, the apoptosis of SGC7901 was assessed with flow cytometry, and the expression level of MMP9,Bax,Akt,P-Akt was tested by Western blotting.Result: The proliferation of SGC7901 cells could not be inhibited by 10 ?mol/L isoliquiritigenin,but it could be significantly inhibited by 25?50and100 ?mol/L isoliquiritigenin,changes were in a concentration-dependent and time-dependent manner, the half inhibitory concentration (IC50) of 24 ? 48 ? 72 h respectively was 52.48?44.49?32.50 ?mol/L,so we chosed 25?50?100 ?mol/L isoliquiritigenin as subsequent experimental drug concentration and 24h as action time . Compared with the control group ( 209.75±9.29 ) ,the membrane cell number of 25mol/L(138.50±10.15)?50 pmol/L (89.50±16.56)?100 pmol/L (45.00±8.08) decreased gradually(P<0.05). Compared with the control group(3.23±0.45)%, the apoptosis rate of25?mnol/L (6.13±0.61)%?50?mol/L(11.7±0.75)%?100?mnol/L(26.6±1.51)%increased gradually ( P<0.05 ).There was no statistically significant difference between groups of the expression level of protein Akt ; The expression level of P-Akt,MMP9 decreased gradually and Bax increased gradually With the increase of the isoliquiritigenin concentration (P<0.05).Conclusion: The proliferation of SGC7901 cells could be inhibited by appropriate concentration isoliquiritigenin.Isoliquiritigenin can obviously inhibit invasion ability of SGC7901 cells,its mechanism may be related to down regulation of the expression signal transduction pathways protein PI3K/Akt and its downsteam protein MMP9.Isoliquiritigenin can significantly induce the apoptosis of SGC7901 cells, its mechanism may be related to down regulation of the expression signal transduction pathways protein PI3K/AKT and up regulation its downsteam protein Bax.Isoliquiritigenin can exert the anti-tumor efficiency through inhibiting proliferation?decreasing invasive potential and inducing apoptosis.