| Objective:Objective to study the variation of intestinal flora disorder,translocation and intestinal mucosal injury in septic young rats,and the changes of serum inflammatory factor TNF-?and the effect of fish oil on the above conditions.Methods:1.The experimental group and the feeding way: 24 weeks SD baby mice to the health of the four weeks,according to the principle of completely randomized divided into: normal control group,model of sepsis and fish oil treatment group(8)in each group.Normal feeding based diet daily for normal group,model group basis of baby mice daily diet,the experimental group is enriched based diet plus 0.5% fish oil to fill the stomach.According to the above methods of feeding 1 week respectively.Model group,treatment group were all made sepsis model,and according to the 5 mg/kg intraperitoneal injection of e.coli lipopolysaccharide(LPS)preparation,three groups continue to such as the feeding way 3 d.2.Specimen collection:moulding at 2 PM on the third day collecting organizations,chest cardiac blood ELISA experimental determination of serum TNF alpha,acquisition and accurately weigh the liver,spleen,kidney,mesenteric lymph nodes,1 g colon contents,closely ligament 8 cm in distance quickly cut about 10 cm ileum segment in the fore Ma Linzhong soak for pathology examination.3.The outcome: To observe the each group intestinal flora(bifidobacterium,lactobacillus,escherichia coli,staphylococcus aureus)and changes in the rate of bacterial translocation and compared;ELISA experiment groups were serum inflammatory factor changes TNF-? values;With hematoxylin and eosin staining method(HE staining),under a microscope in each group(each group randomly 2)only baby rats ileum group to observe the pathological change.Results:1.General performance: pathological manifestations had sepsis model group after the mold: poor spirit,activity,eating less,chills,piloerection,tachypnea,cyanosis.A few rats are visible when laparotomy peritoneal effusion,part of the intestinal swelling,blackening.Matched with the model group,the pathological changes of the fish oil treatment group were relieved,and the normal control group had no pathological manifestations.2.Sepsis were existence state of intestinal flora disturbance,in the specific quantity for gut bifidobacteria and lactobacillus decreased obviously(P < 0.01),the number of escherichia coli and staphylococcus aureus were significantly increased(P < 0.01),fish oil to sepsis were flora disorder has certain corrective action(P < 0.01).It increases the intestinal bifidobacteria,lactobacilli number(P < 0.01),decreased the number of escherichia coli and staphylococcus(P < 0.01),reduce the occurrence of gut bacteria translocation.3.Serum inflammatory factor TNF-? was significantly increased in septic young rats(P<0.01),fish oil can significantly reduce TNF-?(P <0.01).4.In septic young rats,there was a damage of intestinal mucosa,fish oil could improve the ileal mucosa injury and the pathological changes of ileum Conclusion:Sepsis were intestinal disorders and translocation,associated with the intestinal mucosa damage,higher serum TNF-? obvious inflammation factor;fish oil intervention can promote the growth of gut bifidobacteria and lactobacillus increase number,inhibit the growth of escherichia coli and staphylococcus aureus reduce number;can reduce the occurrence of bacterial translocation;cn the serum TNF-? release has certain inhibition,could reduce the series of tissue and organ damage.Improved the sepsis were the ileum mucosa injury and histopathological changes the ileum,and protect the integrity of the intestinal immune barrier,improve the intestinal mucosal repair. |
PDF Full Text Request