The Effect Of Gastric Cancer Cells On Regulating ThPOK Protein Expression Of CD4+T Cell And The Relation Between It With Differentiation Of CD4+T Lymphocyte Subgroup

Objective:Our study foused on the effect of gastric cancer cells on the expression of ThPOK in CD4+T lymphocytes and the relation between it with differentiation of CD4+T lymphocyte subgroup.Methods:1.Gastric cancer cell line(SGC7901 or BGC823)co-cultured direct or co-cultured indirect with CD4+T cell line(Jurkat T Lymphocyte line)for 6h.Then the Jurkat T Lymphocytes were separated,and cultured for 12 h.Finally we checked the differential expression of ThPOK?IFN-??IL-4?IL-17?FoxP3 in each group.2.We silenced the expression of ThPOK by Lentivirus interference technology(constructing siThPOK-Jurkat cell line),then we checked the differential expression of ThPOK?IFN-??IL-4?IL-17?FoxP3 under different states.Results:1.SGC7901 co-cultured direct with Jurkat Lymphocytes in different proportions,with the increasing of lymphocyte proportion,the expression of ThPOK mRNA rised up(P<0.05).2.And if the gastric cancer cells and Jurkat T lymphocytes were transformed into co-culture(directly or transwell),there was no significant difference of ThPOK expression in transwell co-culture group.It was suggested that the gastric cancer cells can promote the expression of ThPOK in Jurkat lymphocytes through directly contacting with them.3.Jurkat T Lymphocytes secreted more IFN-??IL-4 after co-culturing direct with gastric cancer cells.Furthermore,it was more significant when the Jurkat cells are activated(P<0.05).There was no significant difference in IL-17 expression in each groups(P>0.05).However,the expression of FoxP3 increased,while it was irrelevant with Jurkat lymphocytes activation(P>0.05).4.The expression of ThPOK mRNA and protein in siThPOK-Jurkat lymphocytes decreased obviously(P<0.05).5.The expression of ThPOK in the siThPOK-Jurkat cells was very low,and the secretion of IFN-? decreased(P<0.05.)After the co-culture of siThPOK-Jurkat cells and gastric cancer cells,the expression of IFN-? was higher than that co-culturing with GES-1 cells(P<0.05).Compared with Jurkat cells co-culturing with gastric cancer cells,the siThPOK-Jurkat cells co-culturing with gastric cancer cells secreted less IFN-?,(P<0.05).The expression of IL-4 was significantly decreasing in the siThPOK-Jurkat cells(P<0.05).Moreover,when the Jurkat cells co-culturing with gastric cancer cells,the expression of IL-4 was not significantly differently from co-culturing with GES-1.Furthermore siThPOK-Jurkat cells showed no significant changes in IL-17(P>0.05.).Similarly,even the siThPOK-Jurkat cells co-cultured with gastric cancer cells,the expression of IL-17 cytokine was not significantly defference(P > 0.05).The expression of FoxP3 in siThPOK-Jurkat cells was significantly decreased(P<0.05).When the co-culture of siThPOK-Jurkat cells and gastric cancer cells,the expression of Fox P3 was higher than that co-culturing with GES-1 cells(P<0.05).Conclusion:The gastric cancer cells promoted the expression of ThPOK in Jurkat T lymphocytes.And the gastric cancer cells affected the specific markers of CD4+T Lymphocyte subgroup through regulating the expression of ThPOK.CD4+T lymphocyte subsets were plastic in peripheral.