Association Between Mirna-binding Site Polymorphisms In DNA Repair Gene RAD52 3'-UTR And Hepatocellular Carcinoma Risk

Objective: To explore the relationship between micro RNA(mi RNA)-binding site polymorphisms in DNA repair gene RAD52 3'-untranslated region(3'UTR)and susceptibility to hepatocellular carcinoma(HCC)in Guangxi,which will be helpful to elucidate molecular mechanisms underlying the development of HCC,and provide the scientific basis for prevention and treatment of HCC.Methods: A hospital-based case-control study was conducted in 1,002 patients with HCC and 1,013 cancer-free matched controls from sample bank(January 2007 to April 2011 in First Affiliated Hospital of Guangxi Medical University and Affiliated Tumor Hospital of Guangxi Medical University.)The genotype of 4 single nucleotide polymorphism(SNP)in DNA repair gene RAD52 3'-UTR mi RNA-binding site(rs1051669 G>A,rs1051672 C>T,rs7301931 T>C,rs7310449 A>G)was conducted by using the Sequenom Mass ARRAY platform.The multivariate Logistic regression analysis was applied to estimate odds ratios(ORs)and 95% confidence intervals(CIs)for the associations between the above four SNPs and susceptibility of HCC.Results: The results of our analyses showed that no significant statistical differences in the distributions of age and sex was found between cases and controls(P>0.05).However,smokers,drinkers,and hepatitis B virus(HBV)infection individuals in cases group are significantly higher than the controls(P<0.05).After adjusted by age,sex,smoking,drinking status and HBV infection,no statistically significant correlations in these SNPs of RAD52 and the risk of HCC were found.Further stratified by age,sex,smoking,drinking status and HBV infection,we found in the female population,compared with the female who carried the CT/CC genotypes of RAD52 rs1051669,those who carried the TT genotype had a reduced risk of HCC,and there were interactions with female(TT vs.CT/CC: OR=0.03,95%CI=0.00-0.62,Pfor interaction=0.03);when compared with the female who carried the GA/GG genotypes of rs1051672,those who carried the AA genotype had a reduced risk of HCC,and there were interactions with female(AA vs.GA/GG: OR=0.03,95%CI=0.01-0.88,Pfor interaction=0.04).While,there was no interaction on the risk of HCC between other SNPs and environmental factors such as age,sex,smoking,drinking and HBV infection(P> 0.05).Conclusions: In the female population in Guangxi,the RAD52 rs1051669 and rs1051672 might contribute to HCC susceptibility,while there was no association in the male population.The results of this study need to be repeated in a large sample of the population.