| Objective:To explore the effect of panax notoginseng saponins on the interface of tendon bone healing after anterior cruciate ligament reconstruction.Methods:Experimental materials and groups:adult New Zealand white rabbits(Beijing Haidian District thriving experimental animal farms)27,male,before the experimental animal exercise good,can run,no joint swelling,limping and other abnormalities.The knee front drawer test was negative.27 New Zealand white rabbits were randomly divided into a)blank control group;b)positive control group;c)experimental group.Manufacturing model Methods:Anesthesia(1 ml/kg)was performed with 3%pentobarbital sodium.Knee flexion,patellar medial approach incision of the joint capsule,exposure to the articular cavity.Patella to the lateral dislocation,knees resection of the subacromial fat pad,exposed toe extensor tendon and intercondylar fossa ligament.Up to the long extensor tendon,in the muscle tendon junction cut off,to retain the femoral condylar point.Toe long extensor tendon ends with 1/0 wire weaving traction as a graft.Anterior cruciate ligament injury model was established by cutting the anterior cruciate ligament.Tibial tunnel entrance in the tibial tuberosity 5mm,the point lies in the anterior cruciate ligament tibial foot India Department.The tibial tunnel,from the intercondylar fossa femoral condyle wall drill femoral tunnel,the point of the anterior cruciate ligament point,the point in the femoral epicondyle near the cortex.Tunnel diameter 2mm.Guided needle traction graft ends,from top to bottom through the femoral tunnel,intercondylar fossa,tibial tunnel.Stretch the graft,traction suture in the tibial tubercle cortical fixation,complete the anterior cruciate ligament reconstruction.Intervention measures of tendon bone interface:Tendon Interfacial Intervention Interventions were conducted in each group of experimental animals after the model was established.Drugs were treated with local injection of the intervention method,after a one-time injection.A)blank control group did not give intervention;b)positive control group given VEGF 1 mg intervention;c)experimental group given Panax notoginseng 1mg intervention.Postoperative treatment:Postoperative treatment of knee joint sterile dressings,bandage bandage.After the operation of the New Zealand white rabbits are divided cage feeding,knee is not fixed,free activities.Observe and record the wound condition and its gait.3 days after surgery intramuscular injection of penicillin 400,000 units.Feeding temperature:22℃ to 24℃,humidity 40%-70%.Specimen slices and staining:Three slices of rabbits were sacrificed at 2,4 and 6 weeks after operation.Three rabbits in each group were included in 6 rabbits,each containing a femoral condyle with a full-length tunnel of the femoral segment.Femoral tunnel full length with femoral condyle specimens,10%formalin fixed,EDTA decalcified,paraffin embedded,sliced slices(thickness of about 5um).All sections were stained with routine HE staining,Masson staining,immunohistochemical type Ⅰ collagen staining,immunohistochemical staining of CD34,immunohistochemical staining of VEGF,immunohistochemical VEGHR staining.Observe indicators:The effects of HE staining,CD34 staining,VEGF staining and VEGHR staining on blood supply of tendon bone were observed.The effect of collagen fibers on the healing of tendon bone was observed by HE staining,masson staining and type I collagen staining.The effect of bone formation on the healing of tendon bone was observed by HE staining and masson staining.Results:The first,Effect of Panax Notoginseng Saponins on Early Blood Supply after Anterior Cruciate Ligament Reconstruction.HE staining Vascular count The results of HE staining blood vessel count after anterior cruciate ligament reconstruction.(56.38 ± 36.55),VEGF group(110.47 ±39.27),panax notoginseng saponins group(79.43 ± 50.49),three groups of P(P<0.05),and the number of vascular anastomoses in the second week of anterior cruciate ligament reconstruction(62.58),the mean rank of VEGF group(121.72),the average rank of panax notoginseng saponins(87.21),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which VEGF group in the anterior cross-reconstruction of the tendon bone healing interface 2 weeks the largest number of vascular count,followed by Panax notoginseng saponins.(79.25 ±29.08),VEGF group(82.38 ± 37.73),Panax notoginseng saponins group(163.78 ±71.74),three groups of P(P<0.05),and the number of vascular anastomoses in the posterior cruciate ligament reconstruction(68.06),the average rank of VEGF group(71.28),the average rank of Panax notoginseng saponins(132.16),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng group 4 weeks before the anterior cross-reconstruction of tendon bone healing interface vascular count the most,followed by VEGF group.(63.08 ±43.37),VEGF group(122.20 ± 77.00),Panax notoginseng saponins group(133.08 ±75.00),three groups of P(P<0.05),and the three groups were significantly higher than those in the control group(P<0.05)(101.3),the average rank of panax notoginseng saponins(110.34),P(Kruskal(P<0.05),and the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins in the anterior cross-reconstruction 6 weeks after tendon bone healing interface vascular count the most,followed by VEGF group.CD34 staining vascular count before anterior cruciate ligament reconstruction of tendon bone healing interface CD34 staining blood count results.(61.67 ± 11.24),VEGF group(127.33 ± 24.58),panax notoginseng saponins(150.33 ± 10.60),three groups of P(P<0.05),and the two groups were analyzed by univariate analysis of variance in the second week of anterior cruciate ligament reconstruction(Levene Statistic)>0.05,the variance is homogeneous,P<0.05,indicating that there is a statistically significant difference between the three groups,of which Panax notoginseng saponins in the anterior cross-reconstruction after tendon bone healing interface 2 weeks the largest number of vascular count,followed by VEGF group The(78.00 ± 1.73),VEGF group(220.67 ± 22.72),panax notoginseng saponins(172.67 ± 16.92),three groups of P(P<0.05),and the number of vascular anastomoses in the posterior cruciate ligament reconstruction(Levene Statistic)>0.05,the variance is homogeneous,P<0.05,indicating a statistically significant difference between the three groups,of which VEGF group 4 weeks before the anterior cross-reconstruction of the tendon bone healing interface vascular count the most,followed by Panax notoginseng saponins The(42.00 ± 3.61),VEGF group(157.33 ± 43.66),panax notoginseng saponins(47.00 ± 12.12),three groups of P(P<0.05),and the number of vascular anastomoses in the posterior cruciate ligament reconstruction(8.00),the average rank of Panax notoginseng saponins(2.00),P(Kruskal(2)),the mean rank of the blank group(5.00),the mean rank of the VEGF group(8.00)-Wallis Test)<0.05,indicating that there was a statistically significant difference between the three groups,of which VEGF group in the anterior cross-reconstruction 6 weeks after the tendon bone healing interface vascular count the most.Evaluation of VEGF staining in tendon bone healing after anterior cruciate ligament reconstruction with VEGF staining.(1.67 ± 0.81),VEGF group(4.67 ±1.51),panax notoginseng saponins group(6.00 ± 0.00),and the three groups(P<0.05)(10.63),the average rank of panax notoginseng saponins(14.00),P(P<0.05),the difference between the two groups(P<0.05),the difference(P<0.05),the variance,Kruskal-Wallis Test,Kruskal-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins in the anterior cross-reconstruction after tendon bone healing interface 2 weeks the highest staining score,followed by VEGF group.(2.33 ± 1.97),VEGF group(5.50 ± 1.23),panax notoginseng saponins(6.17 ± 1.60),three groups(P<0.05),and the three groups were treated with VEGF P(0.05).The difference was statistically significant between the three groups(P<0.05),and the difference was significant(P<0.05),and the difference was statistically significant between the three groups.group.(2.83 ± 1.60),VEGF group(5.33 ± 1.03),panax notoginseng saponins(7.50 ± 1.64),three groups of P(P<0.05),and the number of vascular anastomoses in the 6th week of the anterior cruciate ligament reconstruction(Levene Statistic)>0.05,the variance is homogeneous,P<0.05,indicating that there is a statistically significant difference between the three groups,of which the total saponins of Panax notoginseng group 6 weeks after the anterior cross reconstruction of tendon bone healing interface vascular staining score,followed by VEGF group.VEGHR staining was used to evaluate the blood vessel count of the tendon bone healing interface after anterior cruciate ligament reconstruction.(4.50 ± 1.23),VEGF group(6.50 ± 1.23),panax notoginseng saponins group(5.67 ± 0.82),three groups of P(P<0.05),and the two groups were significantly higher than those in the control group(P<0.05)(Levene Statistic)>0.05,the variance is homogeneous,P<0.05,indicating that the three groups were statistically significant,in which VEGF group in the anterior cross-reconstruction after tendon bone healing interface 2 weeks staining score,followed by Panax notoginseng saponins The(3.67 ± 1.51),VEGF group(5.33 ± 1.03),panax notoginseng saponins(6.00 ±0.00),three groups of P(P<0.05),and the difference was statistically significant(13.7),the average rank of the VEGF group(10.33),the average rank of Panax notoginseng saponins(13.00),P(Kruskal(P<0.05),the difference between the two groups-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins group before the anterior cross-reconstruction 4 weeks tendon bone healing interface staining score,followed by VEGF group.(5.33± 1.03),VEGF group(7.00 ± 1.55),panax notoginseng saponins group(8.00 ± 1.64),three groups of P(P<0.05),and the three groups were significantly higher than those in the control group(P<0.05)(5,100),the mean rank of the VEGF group(10.67),the average rank of Panax notoginseng saponins(12.00),P(Kruskal-Wallis Test)>0.05,indicating that there was no statistically significant difference between the three groups.The second,Effect of Panax Notoginseng Saponins on Early Collagen Fibers after Anterior Cruciate Ligament Reconstruction.HE staining of collagen fibers before anterior cruciate ligament reconstruction of tendon bone healing interface HE staining of collagen fibers.(24.98 ± 13.70),VEGF group(28.70 ± 13.96),panax notoginseng saponins(24.10 ± 8.98),three groups of P(P<0.05),and the two groups were significantly higher than those in the control group(P<0.05)(85.68),the average rank of VEGF group(99.61),the average rank of panax notoginseng group(86.82),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)>0.05,indicating that there was no statistically significant difference between the three groups,indicating that there was no significant difference in collagen fibers between the two weeks after the anterior cross-reconstruction.(22.62 ± 9.79),VEGF group(25.23 ± 10.04),panax notoginseng saponins group(29.00 ± 11.41),three groups of P(P<0.05),and the changes of collagen fibers in the posterior cruciate ligament reconstruction(Levene Statistic)>0.05,the variance was homogeneous,P<0.05,indicating that there was statistical significance between the three groups,of which Panax notoginseng saponins group 4 weeks before the anterior cross-reconstruction of tendon bone healing interface collagen fibers up,followed by VEGF group The(14.70 ± 4.97),VEGF group(27.10 ± 11.56),panax notoginseng saponins group(27.30 ± 6.22),three groups of P(P<0.05),and the three groups were analyzed by univariate analysis of collagen fibers in the anterior cruciate ligament reconstruction(43.63),the average rank of VEGF group(108.68),the average rank of panax notoginseng saponins(119.29),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng group 6 weeks before the anterior cross-reconstruction of the tendon bone healing interface collagen fibers up,followed by VEGF group.I-type collagen fibers before the anterior cruciate ligament reconstruction of the tendon bone healing interface type Ⅰ collagen staining collagen fibers.(29.03 ± 5.59),VEGF group(37.53 ± 5.14),total saponins of panax notoginseng(49.43 ± 9.72),tris(20.37),the average rank of VEGF group(45.55),the average rank of panax notoginseng saponins(70.58),P(Lev)(Kruskal-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins in the anterior cross-reconstruction of the tendon bone healing interface 2 weeks collagen fibers up,followed by VEGF group.(36.67 ± 6.35),VEGF group(43.70 ± 9.46),Panax notoginseng saponins group(51.07 ± 12.90),three groups of P(P<0.05)(48.63),the average rank of Panax notoginseng saponins(60.07),P(Kruskal),the mean rank of the blank group(27.80),the average rank of the VEGF group(48.63)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng group 4 weeks before the anterior cross-reconstruction of the tendon bone healing interface,the most collagen fibers,followed by VEGF group.(48.63 ± 7.43),VEGF group(72.87± 12.41),panax notoginseng saponins(79.37 ± 12.96),three groups of P(P<0.05),and the difference was statistically significant(P<0.05)(18.32),the average rank of VEGF group(53.32),the average rank of panax notoginseng saponins(64.97),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng group 6 weeks before the anterior cross-reconstruction of the tendon bone healing interface collagen fibers up,followed by VEGF group.Masson staining of collagen fibers before anterior cruciate ligament reconstruction of tendon bone healing interface VEGHR staining of collagen fibers in Table 13 to Table 14.(26.60 ± 5.00),VEGF group(44.43 ± 7.33),panax notoginseng saponins(51.07 ± 8.95),three groups of P(P<0.05),and the two groups were significantly higher than those in the control group(P<0.05)(36.68),the average rank of VEGF group(53.55),the average rank of panax notoginseng saponirns(66.67),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins in the anterior cross-reconstruction after tendon bone healing interface 2 weeks collagen fibers up,followed by VEGF group.(32.23 ± 4.70),VEGF group(46.23 ± 9.52),panax notoginseng saponins(56.43 ± 11.99),three groups of P(P<0.05),and the changes of collagen fibers in the posterior cruciate ligament reconstruction(18.90),the average rank of VEGF group(50.65),the average rank of panax notoginseng saponins(66.95),P(Kruskal&It;RTI ID = 0.0>)&It;/RTI>-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng group 4 weeks before the anterior cross-reconstruction of the tendon bone healing interface,the most collagen fibers,followed by VEGF group.(42.90 ± 6.78),VEGF group(58.43 ± 10.79),Panax notoginseng saponins group(63.83 ± 9.80),three groups of P(P<0.05)(50.00),the average rank of VEGF group(53.08),the average rank of Panax notoginseng saponins(63.02),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng group 6 weeks before the anterior cross-reconstruction of the tendon bone healing interface collagen fibers up,followed by VEGF group。The third,the effect of panax notoginseng saponins on early bone formation after anterior cruciate ligament reconstruction.HE staining of bone anterior cruciate ligament reconstruction of tendon bone healing interface HE staining bone formation results in Table 15-Table 16.(21.12 ±8.16),VEGF group(28.48 ± 16.44),Panax notoginseng saponins group(44.50 ±17.90),three groups of P(P<0.05),and the two groups were significantly higher than those in the control group(P<0.05)(57.33),the mean rank of the VEGF group(82.3),the average rank of Panax notoginseng saponins(131.21),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins in the anterior cross-reconstruction of the tendon bone healing interface 2 weeks the largest bone formation,followed by VEGF group.(36.68 ± 9.40),VEGF group(43.10 ± 10.35),panax notoginseng saponins group(59.67 ± 15.09),three groups of P(P<0.05),and the three groups were significantly higher than those in the control group(P<0.05)(56.93),the average rank of VEGF group(81.58),the average rank of panax notoginseng group(132.99),P(Kruskal&It;RTI ID = 0.0>-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins group before the anterior cross-reconstruction 4 weeks tendon bone healing interface bone formation,followed by VEGF group.(50.25 ± 8.87),VEGF group(67.68 ± 15.07),panax notoginseng saponins group(76.02 ± 18.67),three groups of P(P<0.05),and the difference was statistically significant(P<0.05)(48.68),the average rank of VEGF group(101.05),the average rank of Panax notoginseng saponins(121.77),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins group 6 weeks before the anterior cross-reconstruction of the tendon bone healing interface bone formation,followed by VEGF group.Masson staining of bone formation before anterior cruciate ligament reconstruction of tendon bone healing interface masson staining bone formation results in Table 17-Table 18.(35.40 ± 4.77),VEGF group(52.67 ± 21.17),panax notoginseng saponins(73.97 ± 14.73),three groups of P(P<0.05),and the two groups were significantly higher than those in the control group(P<0.05)(42.98),the average rank of VEGF group(42.88),the average rank of panax notoginseng saponins(68.68),P(Kruskal(P<0.05),the difference between the two groups-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins in the anterior cross-reconstruction of the tendon bone healing interface 2 weeks the largest bone formation,followed by VEGF group.(45.83 ± 9.12),VEGF group(66.73 ± 12.9),panax notoginseng saponins(74.93 ± 14.43),three groups of P(P<0.05),and the changes of bone formation in the first 4 weeks after the anterior cruciate ligament reconstruction(Levene Statistic)>0.05,the variance is homogeneous,P<0.05,indicating that there is a statistically significant difference between the three groups,of which the total saponins of Panax notoginseng group 4 weeks before the anterior cross-reconstruction The(54.03 ± 11.00),VEGF group(67.03 ± 25.04),panax notoginseng saponins group(100.83 ± 21.90),three groups of P(P<0.05),and the three groups were significantly higher than those in the control group(P<0.05)(40.67),the average rank of VEGF group(40.17),the average rank of panax notoginseng saponins(70.67),P(Kruskal(P<0.05),the difference between the two groups(P<0.05)-Wallis Test)<0.05,indicating a statistically significant difference between the three groups,of which Panax notoginseng saponins group 6 weeks before the anterior cross-reconstruction of the tendon bone healing interface bone formation,followed by VEGF group.Conclusion:First,panax notoginseng saponins on the anterior cruciate ligament reconstruction of the early tendon bone healing interface blood supply to promote the role.The effect of Panax notoginseng saponins on the angiogenesis of the anterior cruciate ligament after the reconstruction of the anterior cruciate ligament was significantly higher than that of the blank group and the VEGF group at the 4th week and the 6th week after operation.The mechanism of action may be related to the promotion of multiple cells Factor secretion,promote neovascularization related.Second,the total saponins of Panax notoginseng can promote the formation of collagen fibers in the early tendon healing after anterior cross-reconstruction.The effect of Panax notoginseng saponins on the formation of collagen fibers at the 2nd week,the 4th week and the 6th week after the operation of the anterior cruciate ligament reconstruction were significantly enhanced compared with the blank group and the VEGF group.The mechanism of action of the total saponins Promote a large number of collagen type I synthesis,and ultimately promote the formation of collagen fibers.Third,the total saponins of Panax notoginseng can promote the formation of bone in the early tendon healing after reconstruction of the anterior cruciate ligament.Panax notoginseng saponins had a significant effect on the formation of bone formation in the second week,the fourth week and the sixth week after the anterior cruciate ligament reconstruction.The mechanism of action was probably related to the promotion Osteoblast growth,inhibition of osteoclast growth. |
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