Research Of Cervical Sympathetic Ganglion And Sympathetic Post-ganglia Fibers Mediated Different Sympathetic Nerve Symptoms

BackgroundThere are some patients with cervical spondylosis accompanied by sympathetic symptoms,such as dizziness,palpitation,tinnitus,blurred vision,nausea and gastrointestinal symptoms which can't be completely explained by cervical spinal cord compression.At present the research of the pathogenesis of this disease is still in groping stage.People propose multiple hypothesis to explain the pathogenesis,Such as Vertebral artery pressure hypothesis,cervical instability hypothesis,humoral stimulation hypothesis and cervical sympathetic nerve stimulation hypothesis.Each hypothesis has some theoretical basis,but also can't perfectly explain the symptoms of all patients.Based on cervical sympathetic nerve stimulation hypothesis,our team run some anatomy and electrophysiology experiment.We confirmed cervical longitudinal ligament is rich made by cervical sympathetic ganglion of the sympathetic nerve fibers,and its distribution has a certain regularity.In clinical practice,we observed that anterior cervical surgery or cervical sympathetic ganglion anesthetic technique,can relieve the sympathetic nerve symptoms in part of the patients.According to the above theoretical basis and clinical experience,we propose a conjecture,different sympathetic symptoms may be related to cervical sympathetic ganglions and their post-ganglia fibers in the posterior longitudinal ligament.ObjectivesLooking for the relationship between sympathetic symptoms and cervical sympathetic ganglions and their post-ganglia fibers in the posterior longitudinal ligament.We choose the gastrointestinal smooth muscle potential and arterial blood pressure in this study.To verify the relationship between cervical sympathetic ganglions and the gastrointestinal smooth muscle potential and arterial blood pressure.MethodsPart OneSelect 10 adult healthy New Zealand rabbits,after intravenous anesthesia,stimulate the superior cervical ganglion(SCG),middle cervical ganglion(MCG),inferior cervical ganglion(ICG)with 50 mv intensity of electrical stimulation.Stimulate SCG with 0 mv electrical stimulating as control group.We collect the gastrointestinal smooth muscle potential waveform diagram,select potential amplitude and slow wave cycle time as the research status.Part TwoSelect 10 adult healthy New Zealand rabbits,after intravenous anesthesia,stimulate the superior cervical ganglion(SCG),middle cervical ganglion(MCG),inferior cervical ganglion(ICG)with 50 mv intensity of electrical stimulation.We stimulate SCG with 0 mv electrical stimulating as control group.We collect the arterial blood pressure waveform diagram,select systolic blood pressure and diastolic blood pressure as the research status.All the results were expressed by x±s.All the statistical relationship among the results were analyzed by IBM SPSS Statistic 21.0.Means among different status in a group were analyzed in paired-samples t test.And that between two different groups were analyzed in Dunnett-t test.We defined the P<0.05 as statistically significant difference.ResultsPart OnePotential amplitude before stimulation:SCG 0.858±0.537 mv,MCG 0.761±0.341 mv,ICG 0.787±0.413 mv,Control group 0.777±0.355 mv.Potential amplitude after stimulation:SCG 0.605±0.315 mv,MCG 0.580±0.359 mv,ICG 0.583±0.273 mv,Control group 0.714±0.301 mv.The potential amplitude decreased with statistically significant difference(P<0.05)after stimulation in SGC group and ICG group.But it was nonsignificant(P>0.05)when compared with Control group.Five slow wave cycle time before stimulation: SCG 39.4±6.57 seconds,MCG 40.5±5.95 seconds,ICG 39.8±8.94 seconds,Control group 38.1±8.99 seconds.Five slow wave cycle time after stimulation: SCG 29.3±7.73 seconds,MCG 32.0±8.96 seconds,ICG 27.1±6.50 seconds,Control group 36.7±8.38 Iseconds.The Slow wave cycle time decreased with statistically significant difference(P<0.05)after stimulation in three groups.But only in ICG group the distinction was significant(P<0.05)when compared with Control group.The distinction in SGC group and MCG group was nonsignificant(P>0.05)when compared with Control group.Part TwoDiastolic blood pressure before stimulation:SCG 91.4±7.3mmHg,MCG 90.8±5.4 mmHg,ICG 90.9±8.2 mmHg,Control group 90.3±7.1 mmHg.Diastolic blood pressure after stimulation: SCG 93.6±7.9mmHg,MCG 88.9±8.3mm Hg,ICG 90.9±7.7mmHg,Control group 91.1±7.2mm Hg.The distinction of Diastolic blood pressure before and after the stimulus was nonsignificant(P>0.05)in all the group.Systolic blood pressure before stimulation: SCG 105.1±12.2mmHg,MCG 103.1±8.7 mmHg,ICG 101.9±14.1 mmHg,Control group 102.1±13.6 mmHg.Systolic blood pressure after stimulation: SCG 108.7±8.7mmHg,MCG 106.2±11.0mmHg,ICG 103.7±14.5mmHg,Control group 103.7±14.9mmHg.The difference of Systolic blood pressure before and after the stimulus was nonsignificant(P>0.05)in four group.ConclusionsThe test results told us that gastrointestinal smooth muscle potential slow wave have corresponding relationship with inferior cervical ganglion.That is to say,stimulation of inferior cervical ganglion can cause gastrointestinal smooth muscle slow wave cycle changes,which in turn affect gastrointestinal peristalsis function lead to gastrointestinal disorders,cause gastrointestinal symptoms,including nausea,diarrhea,constipation.There is no evidence that CSG associated with artery blood pressure.