After-effects And Physiological Electricity Changes Of AD Rats By TDCS

Alzheimer's disease(AD)is a progressive central nervous system degeneration disease,the most important manifestations of clinical include learning and memory disorders,cognitive decline and language barriers.With the old people of the society are becoming more and more,the number of AD patients is also a blowout growth,the Alzheimer's Disease International(ADI)reported that the global AD patients will generally increase one AD patient every 4 seconds,and the number of AD patients will reach 130 million in 2050.Alzheimer's disease has become a serious social health problem,but still donot have an ideal treatment.Transcranial direct current stimulation(tDCS)as a noninvasive brain stimulation technology,the great potential in the treatment of AD has increasingly been proven,and it have many advantages,such as portable,noninvasive,low cost,and high safety,so tDCS has great value of research.The exploration of using tDCS to treat AD has just begun,there is a lot of experimental exploration space,so a lot of animal experiments and clinical trials need to explore.In the first of this paper,the methods of making AD model rats was be described,and we successfully made AD rats by the method of injecting A?,and the experimental method and safety of tDCS in rats were introduced in detail.Then,the paper combining with behavioral and histopathology explored the after-effects of tDCS treatment in AD rats.After a course of tDCS(2 weeks)treatment,the learning and memory abilities of the rats were measured by morris water test after the end of the treatment,one month after the end of the treatment,two months after the end of the treatment.The brain was perfused after all the morris water tests ended,and use Nissl staining,ChAT and GFAP immunohistochemistry to observe changes of the hippocampal tissues.The results of behavioral test showed that,the learning and memory ability of the tDCS group was still higher than that of the AD model group after 2 months after the end of tDCS treatment.The results of ChAT and GFAP immunohistochemistry showed that: compared to the AD model group,the tDCS group's expression of ChAT-positive cells was increased,the expression of GFAP-positive cells was decreased,and there was significant difference between the two groups after 2 months after the end of tDCS treatment,so that a course of tDCS treatment for AD had at least 2 months Long-term effect,the value of tDCS treatment for AD had been more affirmed.Finally,the effect of tDCS on Motor evoked potentials(MEP)and EEG was also been explored in this paper.The experiment results of the effect of tDCS on the MEP showed that: the anodes tDCS increased the amplitude of MEP and enhanced the excitability of the cortex,with the number of tDCS times increased,the repetitive anodal tDCS can increase cortical excitability more longer times,and that maybe one of the main reasons of why tDCS can improve AD learning and memory ability.The experiment result of the effect of tDCS on the EEG of AD rats showed that: the EEG of AD rats had a small fluctuation after the tDCS,but there was no significant change.