| Objective:Gastric cancer is one of the most common malignant tumors in the world with the incidence in the fourth.It is also the second leading cause ofworldwide cancer mortality.Gastric adenocarcinoma is the main pathological type of gastric cancer,mainly comes from the gastric mucosa gland,accounting for 95% of the cancer of the stomach.Because of gastric adenocarcinoma is not sensitive to radiation and chemotherapy,surgical resection is the main treatment,yet the recurrence rate still remains high.Moreover,the patients with advanced gastric cancer always lost the possibility of surgery.Therefore,it is important to find effective biomarkers to lead early diagnosis and treatment for gastric cancer.Holliday junction recognition protein is a novel protein in recent years,playing a role in cell mitosis and DNA damage repair.The previous research had found that HJURP was overexpressed in many types of cancer,such as non-small cell lung cancer,breast cancer and glioma.This study is to observe the expression of HJURP in gastric adenocarcinoma tissues and para-cancerous tissues,and explore the relationship between the expression of HJURP and clinical pathological features of the patients,and then discuss the clinical significance of HJURP in gastric adenocarcinoma.It is noteworthy that an increasing number of young people are gain gastric adenocarcinoma.Methods : Fifty cases which were pathologically proved to be gastricadenocarcinoma were selected.The expression of HJURP in gastric adenocarcinoma tissues and para-cancerous tissue were measured by immunohistochemistry.We statistically studied whether there was a different expression of HJURP between gastric adenocarcinoma tissues and para-cancerous tissues;then we further analyzed the relationship between HJURP and patients' sex,age,tumor size,tumor differentiation and TNM stage.Meanwhile,using western blot to prove HJURP is differently expressed between gastric adenocarcinoma tissues and normal gastric tissues.SPSS software was used to analyze the result with the T-test and chi-square test.P<0.05 was for statistical significance.At last,survival analysis in Kaplan-Meier database was to explore the influence of HJURP on the prognosis if gastric cancer.Results: 1.The positive rate of HJURP expression in gastric adenocarcinoma was 64.0%(32/50).While the positive rate of HJURP in para-cancerous tissues was28.0%(14/50).There was a higher positive rate in tissues of HCC than that inpara-cancerous tissues.The difference between two groups was statistically significant(P<0.001)by chi-square test.2.The positive expression rate was 82.6% in patients of III-IV stage,which is higher than the positive expression rate in patients of I-II stage with48.1%.The difference was statistically significant(P<0.05).While,there was no significant correlation between the expression of HJURP in gastric cancer and the sex,age,tumor size,tumor differentiation(P>0.05).3.Western Blot showed that the average gray level was 1.14±0.18 in gastric cancer group while the average gray level was0.67±0.07 in control group.The difference was statistically significant(P<0.05).4.The difference of survival rate was no statistically significant in 876 patients of gastric cancer(P>0.05).However,among the patient without metastasis,patients expressing HJURP in a high level had a long survival time than those who were in a low level of HJURP(P<0.05).Conclusions:1.HJURP is overexpressed in gastric adenocarcinoma tissues and isrelated to TNM stage of gastric cancer,suggesting that HJURP may be involved in the development of gastric adenocarcinoma;2.Patients who expressed more HJURP had a long survival time,indicating that HJURP may become a new biomarker for the prognosis of gastric adenocarcinoma. |
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