Correlation Analysis Of LRP5 Gene Q89R Site Polymorphisms And Osteoporosis In The Middle Aged And Old People In Shihezi Area

Objective The aim of this study was to investigate the relationship between the polymorphism of LRP5 gene Q89R site and Osteoporosis among middle aged and old people in Shihezi area.Methods Q89R genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)in 222 middle aged and old people in Shihezi.The genotypes of LRP5 gene Q89R site were classified and the allele frequency distribution was calculated.Biochemical indicators were detected by automatic biochemical analyzer.The Bone mineral density(BMD)of lumbar spine L1-L4 and femoral neck,Ward's triangle area,Troch and femoral shaft were measured by dual X-ray absorptiometry(DXA).Results There were no significant differences in BMD and biochemical parameters in LRP5 gene Q89R site in the middle aged and old male population.The total gene frequencies of QQ,QR and RR genotypes in LRP5 gene Q89R site in the middle aged and old female population were 82.14%,16.97%,0.89%;the normal bone mass group were 88.10%,11.90%,0%;The low bone mass group were 90.24%,9.76%,0%,osteoporosis group were 62.07%,34.48%,3.45%.There was significant difference on gene frequency between osteoporosis group and osteopenia group,normal control group in the comparision of QQ and QR genotype.There was correlation between bone mineral density of lumbar spine L1-L3,Troch and Q89R genotype;No correlation was found between the bone mineral density of lumbar spine L4,Ward 's triangle,femoral neck,femoral shaft and Q89R genotype.Serum phosphorus was significantly correlated with genotype in biochemical parameters.Conclusion There are ethnic and regional differences on the polymorphism of LRP5 gene Q89R site.LRP5 gene Q89R site polymorphism is a predictor of osteoporosis among middle-aged and elderly women in Shihezi area,suggesting that the LRP5 gene is one of the candidate genes that affect bone density.