MiR-192-3p Promotes The Proliferation, Migration And Invasion Of Ovarian Cancer Cells Through Targeting CCNDBP1

Backgrounds Ovarian cancer is one of the common malignant tumors of female genital organs,with high morbidity rate and mortality rate ranks first in all kinds of gynecological tumors.It is dim for clinical symptoms,and difficult for early diagnosis,so a serious threat to women’s phsical health.miRNA,also known as micro RNA,21-23 nt,is one of the widely exists in eukaryotic RNA molecules that can be combined with m RNA,inhibit the transcription of m RNA,so plays an important role in a series of important process of life activites in animals and plants,including substance metabolism,cell cycle,cell differentiation,apoptosis.In the previous study,we used miRNA expression profile to screen out the differentially expressed miRNAs after overexpression of VPS33 B.By identifying and screening,miR-192-3p was selected as the subject of this study.Objections 1.Identify the role of miR-192-3p in ovarian cancer cells.2.To explore the mechanism by which miR-192-3p in ovarian cancer cells.3.To explore the target of miR-192-3p in ovarian cancer cells and its regulatory mechanism.4.To investigate the role of miR-192-3p in overexpressing VPS33 B ovarian cancer cells.Methods 1.Identify the role of miR-192-3p in ovarian cancer cells.(1)Effects of miR-192-3p mimics transfection on proliferation,migration and invasion of ovarian cancer cells.(2)Transfection of miR-192-3p inhibitors for functional recovery experiments.2.To explore the mechanism by which miR-192-3p in ovarian cancer cells.Cell cycle and EMT related proteins was examined by Western Blot.3.To explore the target of miR-192-3p in ovarian cancer cells and its regulatory mechanism.(1)The target gene of miR-192-3p was predicted by bioinformatics software and verified by dual-luciferase reporter assay.(2)Cell functions were used to detect the effect of target gene on ovarian cancer cells.(3)Cell functions were used to detect the effect of target gene on the expression of miR-192-3p mimics in ovarian cancer cells.4.To investigate the role of miR-192-3p in overexpressing VPS33 B ovarian cancer cells: transiently transfection miR-192-3p mimics and VPS33 B plasmids,cell level function detection the effect of miR-192-3p in overexpressing VPS33 B ovarian cancer cells.Results 1.miR-192-3p promotes the proliferation,invasion and migration of ovarian cancer cells.2.miR-192-3p promoted the expression of cell cycle and partial EMT-related proteins in ovarian cancer cells.3.miR-192-3p promotes the proliferation,invasion and migration of ovarian cancer cells by targeting CCNDBP1.4.miR-192-3p part of the participate in VPS33 B overexpressing ovarian cancer cells.Conclusions miR-192-3p plays an important role in carcinogenesis in ovarian cancer and may promote the proliferation,invasion and migration of ovarian cancer cells by targeting CCNDBP1.