| Daphniphyllum calycinum Bench is for Daphniphyllaceae Daphniphyllum species.Polygonum hydropiper Linn is for Polygonum species.Feng Liao was composed of P.hydropoper L.and D.calycinum Bench,which belonged to Chinese Traditional prescription and It was clinically applied for the treatment of dysentery,acute gastroenteritis,traumatic swelling.It was proved that the total flavonoids were the main components of P.hydropoper L.and D.calycinum Bench.This paper was aimed at optimizing the preparation process of FengLiao granule,establishing the quality standardization and evaluating the pharmacological activities.The contents of this paper was as followed:(1)The total flavoniods in FengLiao were determined by UV-visible Spectrophotometry and NaNO2-AlCl3-NaOH system,which takes rutin as reference substance,the standard curve regression equation is y=12.57x-0.0047,R2=0.0097,the good linear ranged from 0~0.040mg/ml;HPLC was used to determine the content of quercetin in FengLiao,the standard curve regression equation is y=43215x-14647,R2=0.9995,the good linear range varied from 10~50μg/ml.(2)The best extraction process and formation technology of FengLiao Granule were optimized by single-factor and orthogonal tests.The optimum preparation condition of alcohol extraction: extracted it with 10 times 65% ethanol for 2 times,60 min in turn.6000 rpm centrifugal it for 10 min.The extract solution was concentrated to1.15~1.25(60℃).Drying it by acuum at 80℃.After that,the granulation condition was selected: 85% ethanol was regarded as wetting agent,the ratio of drug and excipients was1:1.5,the excipients were dextrin and sucrose,and the ratio of them was 3:2,and the drying temperature was 60℃.Based on the above results,quality control of semi-finished produce were discussed by some small experiments.(3)Making out the quality standards by TLC and HPLC,which were established to identify the FengLiao Granule.TLC regards quercetin as reference substance,spotted on polyamide TLC plate,n-butanol-sodium dodecyl sulfate-hexane-water-formic acid(0.63:0.27:0.1:3:0.2)as the agent,3% AlCl3 chromogenic reagent.The total flavonoids and quercetin were determined by UV spectrophotometry and HPLC,the methodology research showed that this method is highly credible and repeatable.it can be used as a quality standard of Fengliao granule.(4)Carrying out the initial pharmacodynamics studies by pain threshold and the relieving pain intensity which measured with acetic acid and thermal stimulation respectively.The results showed: Compared with normal saline group,the hot plate method:the threshold of 15 min pain was significantly prolonged in the high dose group(P < 0.05);Acetic acid writhing method: high and middle dose of alcohol can significantly reduce the number of writhing times(P<0.05);Ear swelling induced by xylene: the swelling rate of alcohol at high dose was significantly increased(P<0.05);the effect of acetic acid on the permeability of capillary in the small intestine of young rats: the absorbance of the high and middle dose groups decreased significantly(P<0.05).(5)The study showed that the preparation technology of FengLiao granule is scientific,reasonable and feasible,the quality standard is controllable and stable.The extract has anti-inflammatory and analgesic effects. |
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