RNF8 Knockout In Mice Cochlea Research On The Effects Of Aging Mechanism

Objective: RNF8 is a kind of ubiquitin ligase E3,play an important role in DNA damage repair,cell apoptosis,aging,mutation reaction and nerve degeneration,the occurrence of tumor.This study through the observation of different type RNF8 knockout(KO)and wild type mice(WT)between the cochlear hair cells,stria vascalris,grain,spiral ganglion cell type,quantity,density,thickness,damage location,such as change,explore RNF8 role in DNA damage repair and RNF8 mice cochlea after missing the main structure of the aging mechanism,provide the basis for presbycusis pathology and treatment.The cochlea based in mice and the embedding technology improvement.Methods: through pair off access to knockout mice and brood of wild.32 weeks to eight weeks,16 weeks,grouping.Using mice cochlea based and embedding tissue section of improving technology,HE,CV staining to observe the cochlea hair cells,stria vascalris,spiral ganglion cells:the change of cell density,quantity,thickness,Immunofluorescence gamma H2 AX dyeing;Immunohistochemical 8-oHdG mark degree and the expression of DNA damage;lipofuscin,beta galactose glucoside enzyme dyeing damaged cell aging test.Results: 1.Mouse cochlear modified materials technology experiment results of time increased by 72.4%,intact rate increased by 41.4%.Slice intact rate 37.2%.2.HE,CV staining,found that age KO group than that of WT hair cells changed obviously.The decrease in the number of stria vascalris,lines appear three layers of cells,cavitation,separation,fracture.Spiral ganglion cells lack clear,serious fracture.3.Cochlear age change found that RNF8 KO mice cochlea hair cells,stria vascalris,grain and increasing the rheological change more obvious,spiral ganglion immunofluorescence gamma H2 AX staining showed damage of cell H2 AX phosphorylation reaction.4.Mouse cochlear aging changes,KO group beta galactose glucoside enzyme staining found vascular pattern change with age sex 32 weeks for lipofuscin staining nuclei are pale brown around.(P<0.05)Conclusion: the improved fast mice cochlea based and slice the embedding technologymethod is simple and easy,low cost,saving resources and is worth promoting.RNF8 is missing mouse cochlear morphological change one of the important reasons,also indirectly affect cochlear function,more and more obvious with increasing age.RNF8 effect on the aging mice cochlea the main structure of differentiation with presbycusis clinical phenotype there are closely related,missing RNF8 accelerated aging mice cochlea.RNF8 loss to mouse cochlear tissue repair function mechanism has an important influence.Mouse cochlear RNF8 defective gene closely related to apoptosis and senescence.