Effect Of The MiR-654-5p/RNF8 Axis On The Epithelial-mesenchymal Transition Of Cancer Cells

Cancer is expected to rank as the leading cause of death and the single most important barrier to increasing life expectancy in every country of the world in the 21 st century.More than 90% of mortality from cancer is attributable to metastases.A key event in promoting stationary tumor cells to migrate and invade is the epithelial�mesenchymal transition(EMT)progress.As an E3 ubiquitin ligase,RNF8 can play an important role in a variety of biological processes,including the formation and progression of tumors.Recent advances have also found that RNF8 plays an important role in breast cancer metastasis.However,due to the heterogeneity of different tumors,the specific role of RNF8 in different cancers are still unclear.Also,the upstream controlling mechanisms of RNF8 in tumor remain to be understood.miRNAs negatively regulate the expression of a large variety of genes both at mRNA and protein level,by degrading their mRNA target and/or by silencing translation,and therefore,participate in important cell biological processes such as cell proliferation,migration,differentiation and apoptosis.Emerging evidence has also suggested the involvement of miRNA in the development and progression of cancer by exerting their regulatory functions through tumor growth,invasion and metastasis,showing a strong functionally overlap with RNF8.In this paper,we investigated the role of RNF8 in the metastasis of liver cancer.We demonstrated that RNF8 was involved in liver cancer metastasis and exerted its function by regulating Erk activity.Furthermore,we explored the upstream mechanism of RNF8 regulation and predicted the miRNA miR-654-5p targeting RNF8 mRNA.Our results confirmed that miR-654-5p can directly target RNF8 and regulate the EMT process of lung cancer.This suggests that miR-654-5p/RNF8 may be a potential target for the treatment of cancer metastasis.