The Effect And Related Mechanism Of Hsa-miR-6841-3p On The Biological Function Of Cervical Cancer Cells

Objectives: In the present study,we firstly examined the expression of miRNA-6841-3p in cervical cancer tissues,and further explore the impact of miRNA-6841-3p on cervical cancer cell viability,proliferation and invasion,then predict and validate the downstream target of miRNA-6841-3p to reveale the potential mechanism of miRNA-6841-3p in cervical cancer.Thus,our study may suggest a potential pathogenesis and develop novel effective therapies for cervical cancer.Methods: The expression of miRNA-6841-3p in human cervical cancer tissues and normal tissues were tested by real-time fluorescent quantitative polymerase chain reaction(RT-PCR).The cervical cancer cell lines CaSki and SiHa were transiently transfected with miR-6841-3p mimics and miR-6841-3p inhibitor by riboFECTTMCP.The cells were divided into four groups: miR-6841-3p mimics group(miR-6841-3p),miR-6841-3p mimics control group(miR-6841-3p-negative control,NC),miR-6841-3p inhibitor group(mi R-6841-3p-in),and miR-6841-3p inhibitor control group(miR-6841-3p-negative control-inhibitor,NC-in).The expression of trefoil factor 3(TFF3)and miRNA-6841-3p in human cervical cancer cell line CaSki and SiHa were detected by RT-PCR.Cell proliferation was evaluated using the Cell Counting Kit-8(CCK-8)assay.Invasion was measured by transwell chamber assays.The wound healing model was used to represent the migration ability.The target gene softwares were used to predict the following target genes of miRNA-6841-3p.TFF3 protein was quantified by Western blot analysis.Results: 1.The expression of miRNA-6841-3p in cervical cancer was significantly lower than that in normal tissues by real-time fluorescence quantitative PCR,and the level was lower in HPV positive tissues than in HPV negative tissues.2.MiRNA-6841-3p mRNA expression in cells transfected with miRNA-6841-3p mimics was significantly higher than that in negative control cells(P<0.01).miRNA-6841-3p mRNA expression in cells transfected with miRNA-6841-3p inhibitor was significantly lower than that in negative control cells(P<0.01).3.The overexpression of miRNA-6841-3p inhibited the viability,and invasion and migration abilities,as shown in the cells transfected with the miRNA-6841-3p mimics(P<0.05).The downregulation of miRNA-6841-3p promoted theviability,and invasion and migration abilities,as shown in the cells transfected with the miRNA-6841-3p inhibitor(P<0.05)4.The expression of TFF3 mRNA decreased in the cells transfected with mimics and increased in the cells transfected with inhibitors.5.When miRNA-6841-3p was overexpression in CaSki and SiHa cells,protein levels of TFF3 was depressed with Western blot,reversely,when miRNA-6841-3p was blocked in those cells protein levels of TFF3 was raised.Conclusion:Our results provide first evidence for the growth suppressive activity of miRNA-6841-3p in cervical cancer,which is largely ascribed to downregulation of TFF3.MiRNA-6841-3p is a promising new target for the development of therapeutic strategies for the clinical treatment of cervical cancer.