Association Of Single Nucleotide Polymorphisms In Vitamin D Receptor Genes With Bone Loss Around Dental Implants

ObjectiveThe purpose of this study is to explore the relationship between VDR gene polymorphism and implant bone loss by analyses of genotypes or allele frequencies.MethodsSubjects were taken from patients referred to the Department of oral planting,the affiliated hospital of Qingdao university Huangdao sorting between January 2011 and November 2016.One hundred and fifty-six patients who had two hundred and fifty dental implants,were divided into four groups:(1)Group A,30 individuals whose implant bone loss was beyond the success criteria of implant success also suffered from periodontitis.50 implants were contained.(2)Group B,58 individuals whose implant bone loss was not beyond the success criteria of implant success just suffered from periodontitis.96 implants were contained.(3)Group C,32 individuals whose implant bone loss was beyond the success criteria of implant success didn’t suffer from periodontitis.44 implants were contained.(4)Group D,bone around implant and periodontal condition of 36 individuals are very well.60 implants were contained.After being collected and purified,the DNA were amplified by PCR and cut by specific extinction enzymes.Restricted DNA products were then separated by 2% agarose gel electrophoresis and the genotypes were judged.Statistical analysis was performed on SPSS20.0 system software.The difference of genotype and the variation of allele frequencies were analyzed by Fisher’s exact test or chi-square test.Results1.The genotypes or allele frequencies of TaqⅠand BsmⅠbetween group A and group BAs for TaqⅠ,the genotypes are TT、Tt、tt.Fisher’s exact test was used to analyze the genotypes between group A and group B.And the results showed that there was no significant differences between the two periodontal groups(p=1.000,p>0.01).Chi-square test was used to analyze the allele frequencies between group A and group B.And the results shows that allele frequencies of TaqⅠwere not significant different between the two periodontal groups(p=0.682,p>0.01).As for BsmⅠ,the genotypes are BB、Bb、bb.Fisher’s exact test was used to analyze the genotypes between group A and group B.And the results showed that there was no significant differences between the two periodontal groups(p=0.890,p>0.01).Chi-square test was used to analyze the allele frequencies between group A and group B.And the results showed that allele frequencies of TaqⅠwere not significant different between the two periodontal groups(p=0.746,p>0.01).2.The genotypes or allele frequencies of TaqⅠand BsmⅠbetween group C and group DAs for TaqⅠ,the genotypes are TT、Tt、tt.Fisher’s exact test was used to analyze the genotypes between group C and group D.And the results showed that there was no significant differences between the two periodontal healthy groups(p=1.000,p>0.01).Chi-square test was used to analyze the allele frequencies between group C and group D.And the results showed that allele frequencies of TaqⅠwere not significant different between the two periodontal healthy groups(p=1.000,p>0.01).As for BsmⅠ,the genotypes are BB、Bb、bb.Fisher’s exact test was used to analyze the genotypes between group C and group D.And the results showed that there was no significant differences between the two periodontal healthy groups(p=0.115,p>0.01).Chi-square test was used to analyze the allele frequencies between group C and group D.And the results showed that allele frequencies of TaqⅠwere not significant different between the two periodontal healthy groups(p=0.226,p>0.01).Conclusions1.For periodontal patients,the genotype or allele of TaqⅠSNP or BsmⅠSNP have no significant effect on the incidence of bone healing.2.For periodontal healthy patients,the genotype or allele of TaqⅠSNP or BsmⅠSNP have no significant effect on the incidence of bone healing.3.The experiment is the base for further research on the relationship between genetics and bone loss around the implants.