| Objectives To investigate the mechanism of apoptosis and the effects of trimetazidine pretreatment on ischemia-reperfusion injury in rats with early pressure,to provide the basic theoretical basis and new ideas for the prevention and treatment for clinical early pressure ulcer.Methods 1 16 Wistar rats were randomly divided into 2 groups: the control group(Sham group),ischemia-reperfusion group(I/R group).I/R group were modeled with special pressure device to prepare rat model of early pressure ulcer.At the end of the experiment,take the local muscle tissue of compression.The pathological changes were observed by HE staining,TUNEL method was used to detect the apoptotic cells of the compressed muscle tissue,the expression of Bax and Bcl-2 were detected by immunohistochemistry,Western blot methods to detect the expression of Bax,Bcl-2 and CHOP in the compressed tissue.2 40 Wistar rats were randomly divided into 5 groups: the control group(Sham group),ischemia-reperfusion group(I/R group),trimetazidine low dose group(TMZ low group),trimetazidine middle dose group(TMZ medium group),trimetazidine in high dose group(TMZ high group).Preparation of rat model of early pressure ulcer with special pressure device.Rats in the I/R group were modeled only,rats in the trimetazidine pretreatment group were given trimetazidine 7 days before modeling 5mg/kg,10mg/kg,15mg/kg intragastric pretreatment.At the end of the experiment,take the local muscle tissue of compression.The pathological changes were observed by HE staining,TUNEL method was used to detect the apoptotic cells in each group,the expression of Bax and Bcl-2 were detected by immunohistochemistry,Western blot methods to detect the expression of Bax,Bcl-2 and CHOP in the compressed tissue.Results 1 Light microscopic observation of compression tissue found,in the Sham group,the muscle fibers tightlypacked,normal morphology,no inflammatory cell infiltration and pathological changes.Compared with the Sham group,there were more serious pathological changes of the muscle fibers in I/R group;TUNEL test was used to detect the apoptotic tissue results showed that,apoptotic cells are rarely seen in Sham group,and the expression of apoptotic cells in the I/R group was significantly increased(P<0.05);The results of immunohistochemistry showed that Bax and Bcl-2 protein were consistent with the trend of Western blot,the Bax protein in the Sham group almost no expression or minimal expression,compared with the Sham group Bax protein expression was significantly increased in I/R group(P<0.05),the expression of Bcl-2 protein was significantly in Sham group,compared with the Sham group Bcl-2 protein expression was significantly lower in I/R group(P<0.05);Western blot detected the expression of CHOPprotein in compressed tissue showed that,in the Sham group almost no expression or only minimal expression,compared with the Sham group CHOP protein expression was significantly increased in I/R group(P<0.05).2 Light microscopic observation of compression tissue found,in the Sham group,the muscle fibers tightlypacked,normal morphology.Compared with the Sham group,there were more serious pathological changes of the muscle fibers in I/R group.The degree of pathologic damage of the muscle fiber in the trimetazidine pretreatment group was attenuated with the dose increasing.TUNEL test was used to detect the apoptotic tissue results showed that,apoptotic cells are rarely seen in Sham group,and the expression of apoptotic cells in the I/R group was significantly increased(P<0.05),but decreased with the dose increasing in the trimetazidine pretreatment group.The results of immunohistochemistry showed that Bax and Bcl-2 protein were consistent with the trend of Western blot,the Bax protein in the Sham group almost no expression or minimal expression,compared with the Sham group Bax protein expression was significantly increased in I/R group(P<0.05),but decreased with the dose increasing in the trimetazidine pretreatment group.The expression of Bcl-2 protein was significantly in Sham group,compared with the Sham group Bcl-2 protein expression was significantly lower in I/R group(P<0.05),but increased gradually with the dose increasing in the trimetazidine pretreatment group.Western blot detected the expression of CHOP protein in compressed tissue showed that,in the Sham group almost no expression or only minimal expression,compared with the Sham group CHOP protein expression was significantly increased in I/R group(P<0.05),but decreased with the dose increasing in the trimetazidine pretreatment group.Conclusions 1 Apoptosis was involved in the formation of early pressure sore in rats.The expression of Bcl-2 protein was decreased and the expression of Bax and CHOP protein increased.2 The pretreatment of trimetazidine has protective effect on ischemiareperfusion injury in rats with pressure ulcer,can reduce the degree of pathological damage of the compressed muscle tissue,increase the expression level of Bcl-2,down-regulation the expression level of Bax and CHOP to inhibit apoptosis,protect the muscle tissue compression.Pretreatment with trimetazidine high dose(15mg/kg)have better protective effect of ischemia reperfusion injury on pressure ulcers in rats than lower dose. |
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