| Objectives Preparation of mXG gel and establishment of rat abdominal cecal injury adhesion model to analyze and evaluate the effect of mXG gel on postoperative adhesion.Methods 1 Firstly,the natural plant polysaccharide xyloglucan(XG)was obtained by isolating and purifing from tamarind gum.Secondly,the modified xyloglucan(mXG)was available by removing partial hydrolysis of part of its side chain through enzymatic reaction.Then,the physical and chemical properties could be characterized by spectrophotometry and scanning electron microscopy.2 The biocompatibility of mXG gel in vivo was evaluated by live/dead cell staining,3D cell culture,and subcutaneous injection of mice.3 Establish the rat abdominal wall-cecal injury adhesive model to evaluate the postoperative anti-adhesion effect of mXG thermo-sensitive gel.48 SD rats were randomly divided into 3 groups respectively,the positive control group A,1m L saline was injected into the abdominal wall and cecum.The commercial chitosan membrane control group B,the 2×3cm2 chitosan anti adhesion membrane was used to cover the wound of abdominal wall.The mXG hydrogel group C,1m L4%(w/v)mXGhydrogel was injected into the abdominal wall and cecal wound,after the complete formation of the hydrogel.8 rats were killed in each group 7 day and 14 day after operation to evaluate the degree of adhesion and histological observation.4 To analyze and evaluate mXG gel on anti-adhesion process by immunohistochemistry.Results 1 The internal structure of mXG gel showed discontinuous porous structure,which was beneficial to the transmission and diffusion of oxygen,water,nutrients and metabolites.2 The mXG gel was not cytotoxic by live/dead cell staining,it had good cell compatibility by 3D cell culture,it showed complete degradation within 6 weeks and histological observation showed the implantation site did not cause other adverse reactions.The above experimental results showed that the mXG gel had good biocompatibility and biodegradability.3 7 day after operation,a more severe adhesion formed in the group A,with fibrous connective tissue hyperplasia.Moderate adhesion formed in the group B with more connective tissue.Most cecum and abdominal wall began to heal in the group C with less connective tissue and some inflammatory cell infiltration.According to Nair five grading standards and histological observation grading,there was statistical significance between group C and group A(P<0.05),there was statistical significance between group C and group B(P<0.05),but there was no statistical significance between group A and group B.14 day after operation,nice recovery of the cecum and abdominal wall can be observed in in the group C,even more severe peritoneal adhesions formed in the control group A,with more fibrous connective tissue hyperplasia.In the film group B,severe adhesion to the control group was formed.The recovery of group B is between group A and C,the difference between the three groups were statistically significant(P?0.05).4 Immunohistochemical results showed that the expression of CTGF and TGF-?1 increased with the increase of peritoneal adhesions and collagen deposition 1 week and 2 week after operation,and the positive expression of CTGF was the lowest in group Cand the highest in group A.Conclusions 1 The mXG solution had excellent temperature sensitivity,and could rapidly form gel with high strength after injection into the wound.2 Cytotoxicity experiments,in vivo degradation experiments and pathology showed that mXG gel had a good biocompatibility.3 The mXG gel had good effect in preventing abdominal adhesions.4 Immunohistochemical study showed that CTGF and TGF-?1 were important cytokines that affected the formation of postoperative peritoneal adhesions. |
PDF Full Text Request