Influence Of Atorvastatin On PEDF Expression In Myocardium And Retina Of Mice With Diabetic Myocardial Infarction

Objective: To discuss whether atorvastatin can adjust angiogenesis of myocardium and retina of mice with diabetic myocardial infarction and to discuss its tissue and organ specificity,speculate its mechanism of action and to provide new theory evidence for its prevention and treatment effect in myocardial infarction and diabetic retinopathy.Methods: 60 healthy adult Wistar rats were selected as the research objects and were divided into four groups: the control drug group,the control nondrug group,diabetic myocardial infarction drug group,and diabetic myocardial infarction non-drug group.STZ enterocoelia and Iso subcutaneous injection were used to establish mice models with diabetes and myocardial infarction and venous blood was taken to prepare plasma for determining CK-MB results.After successful modeling,gavage of atorvastatin suspension was given to mice in the drug group according to weight while gavage of normal saline of the same volume was given to mice in the non-drug group.After 3 months of intragastric administration,the blood glucose of mice in the diabetic myocardial infarction group was detected;mice with blood glucose lower than 14mmol/L were removed and the resting survival and effective mice in each group were counted and killed after chloral hydrate anesthesia.Five mice were selected from each group for detection of PEDF expression in myocardium and retina of mice,which was detected by using western-blot method,and the rest mice were used for histopathology detection: myocardium and retina were taken to prepare sections to observe angiogenesis condition.Results: 5min,10 min and 30 min after Iso injection,electrocardiogram of mice in the diabetic myocardial infarction group were significantly changed,with elevated hollow back in ST segment and with arrhythmia in some cases;after reperfusion 3h and reperfusion 20 h,serum CK-MB level increased significantly compared with that before Iso injection and the difference had statistical significance(P<0.05).Compared with the control group,myocardium PEDF protein expression of mice in the diabetic myocardial infarction non-drug group increased significantly after myocardial infarction(P<0.05),PEDF protein expression in the retina decreased significantly(P<0.05),and the differences had statistical significance;compared with non-drug group,myocardium PEDF protein expression of mice in the diabetic myocardial infarction drug group decreased relevantly(P<0.05)and PEDF protein expression in the retina increased relevantly(P<0.05),the difference had statistical significance.Angiogenesis condition in the rat myocardium and retina was observed microscopically.Compared with the control group,retinal neovascularization was more evident in the diabetic myocardial infarction non-drug group and there was little small vessels in myocardial tissue;compared with diabetic myocardial infarction non-drug group,retinal neovascularization number in the drug group decreased relevantly and connected normal myocardial cells can still be seen in the myocardium,with a large amount of new small vessels distributed in it.Conclusion:(1)Statin can influence angiogenesis of mice with diabetic myocardial infarction via the expression of myocardium retina PEDF;(2)The influence of statin on angiogenesis is different in different organs and different tissues,namely,it has organ tissue specificity.