The Expression And Function Of Minichromosome Maintenance7 In Pituitary Adenoma

Objective:In this study we used immunochemistry to detect MCM7 protein expression in pituitary adenoma.Furthermore,MCM7 and their clinic pathological significance of pituitary adenoma have been explored.Methods: Eighty cases of surgically resented pituitary adenoma specimens were collected from HeBei province the second hospital from2015.11 to 2016.11.A11 specimens had been confirmed by clinical and pathological diagnosis.The patients were classified histological as 20 cases of PRL adenoma,20 cases of GH adenoma,40 cases of NF adenoma.According to the Knops classification,29 cases belong to invasive pituitary adenoma,51 cases belong to noninvasive pituitary adenoma.Using immunochemistry,we measured MCM7 in tumor tissue from patients with pituitary adenoma.Results:1 In immunochemistry,MCM7 protein can not be detected in normol pituitary tissue.2 In immunochemistry,MCM7 protein was detected in cytoplasm and nucleus.The score of MCM7 was the highest in NIPA(6.11±2.89),as compared with other tumors.IPA exhibited the lowest expression levels,thus the comparison with other three tumor subtypes demonstrates a great statistical significance(P<0.05).The expression of MCM7 had no statistical differences in different sex,tumor Size and invasiveness(P>0.05).3 In immunochemistry,MCM7 protein was detected in cytoplasm and around the nucleus.Conclusions:1 MCM7 protein were all detected in pituitary adenoma.2 The expression level of MCM7 are higher in NIPA and function pituitary adenoma.3 In conclusion,in this work we supposes that Mcm7 is a novel P53 interacting protein.Mcm7 binds to P53 and inhibits P53 mediated transcription.