| Objective: Nowadays our country has about 3 million patients with corneal blindness,and the annual growth rate of 10-20 million.Corneal transplantation is the most effective treatment for patients with corneal blindness.And corneal neovascularization is still an important risk factor for corneal transplantation.At present glucocorticosteroids is the main kind of drug to control corneal neovascularization after corneal transplantation in clinical.It is with the well efficacy easily for controlling corneal neovascularization.But it easily lead to hormone glaucoma,cataract and other complications.It is important to have a safe and effective anti-neovascular drug that can effectively control the growth of corneal neovascularization after corneal transplantation and rarely cause eye complications.Conbercept is a new kind of anti-VEGF(Vascular endothelial growth factor)drug developed by our country with independent intellectual property rights.It has been widely used in the treatment of choroid and retinal neovascularization.At present,there are few reports on the application of Conbercept to neovascularization after corneal transplantation.In this study,we observed the effect of Conbercept on rabbit corneal transplantation and further explored its therapeutic effect on neovascularization after penetrating keratoplasty(PKP).It is well known that the use of vascular growth inhibitors in vascularized tissue will affect tissue wound healing processes.Whether Conbercept is used to control corneal neovascularization will hinder the healing of corneal trauma is an important issue in this experiment.After the cornea is damaged,the static corneal stromal cells are activated into corneal fibroblasts.During the repair process corneal fibroblasts are further transformed into morphologically and functionally distinct myofibroblasts cells.Myofibroblasts and smooth muscle cells have the same characteristics,with contraction,can promote wound healing.?-smooth muscle actin(?-SMA)is the specific cell marker of corneal myofibroblasts.Itcan reflect the levels of myofibroblasts in the cornea,and presumably the healing degree of corneal wound.The aim of this study was to investigate the effect of Conbercept on neovascularization after penetrating keratoplasty in rabbits by measuring the expression of VEGF,and to observe the changes in the expression of factor ?-SMA for speculating the heal degree of corneal wound of rabbits and the effect of Conbercept on wound healing.Thus reach the purpose of guiding to control neovascularization after human penetrating keratoplasty.Methods:1 Group: 48 New Zealand white rabbits were randomly divided into four groups(n = 12): control group,experimental group 1,experimental group 2 and experimental group 3.The control group: routine eye drops,that is,topical tobramycin eye drops was installed four times daily after penetrating keratoplasty(PKP)in rabbits;experimental group 1: routine eye drops,while the addition of topical 0.01% Conbercept four time daily;experimental group 2: routine eye drops,while the addition of topical 0.1% Conbercept four time daily;experimental group 3: routine eye drops,and 0.1% Conbercept(0.1ml)subconjunctivally injection one time every week.2 Preparation of rabbit penetrating keratoplasty model: trephine with a diameter 7.5mm prepared the graft;trephine with a diameter 7.25 mm drilling,with 15°anterior chamber wearing the bayonet corneal fast through,,corneal scissors cut cornea made of bed;at first 10-0 nylon line 12: 00,6: 00,9: 00,3: 00 each stitch 1 needle,and then in each quadrant plus sewing 2-pin,suture graft.48 rabbits in the right eye according to the above steps to prepare the penetrating keratoplasty model.Postoperative eye surgery were given antibiotics eye.Next,drugs were given in groups.3 Real-time quantitative quantitative polymerase chain reaction(qPCR)Three rabbits were sacrificed at at the time of the second week,the fourth week,the sixth week and the eighth week after operation.The expression of VEGF and ?-SMA in the cornea were measured by qPCR method.4 SPSS21.0 software for statistical analysis.Results:1 Expression of VEGF: The expression of VEGF in the control group and each experimental group increased first and then decreased with time,and the expression level of VEGF was the highest at the 4th week.The expression of VEGF was decreased at all time points compared with the control group(P <0.05).There was no significant difference in the expression of VEGF between the experimental group 1 and the experimental group 2(P> 0.05).The expression of VEGF in the experimental group 3 was lower than that in the experimental group 2,and there was no statistically significant difference(P> 0.05).2 Expression of ?-SMA: All the gtoups of ?-SMA showed an increasing trend with time,and there were significant differences between the two groups at all time points(P <0.05).The expression of ?-SMA in all the experimental groups was lower than that in the control group at four time points,and the difference was not statistically significant(P> 0.05);There was no significant difference in the expression of ?-SMA at each time point(P> 0.05)compared with experimental group 2 and experimental group 2.Conclusions:1 Conbercept can inhibit the formation of neovascularization after penetrating keratoplasty in rabbits.2 Conbercept in the inhibition of rabbit penetrating keratoplasty after neovascularization without affecting the healing of corneal wound.3 There was no significant difference in the effect of different Conbercept concentration topically on the neovascularization after penetrating keratoplasty in rabbits.The inhibitory effect of different administration methods on the growth of neovascularization was further confirmed. |
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