The Inhibitory Effect And Its Mechanism Of IP6 + Ins On The Growth Of Ovarian Cancer SKOV3 Cell Line

Objective: In this study,the inhibitory effect of IP6+Ins and its combination with cisplatin on the growth of epithelial ovarian cancer cells were studied from the cell and molecular level,and we aimed to explore the mechanism of its related mechanisms.It can provide the theoretical basis and experimental basis for the treatment of the epithelial ovarian cancerMethods:1 Cell culture:Ovarian cancer SKOV3 cells placed in RPMI 1640 medium containing 10% neonatal bovine serum using cell culture technology.They were Cultured in a 5% CO2 incubator at 37?.2 Experimental grouping: The experiment was randomly divided into six groups: blank control group,IP6+Ins low dose,IP6+Ins middle dose group,IP6+Ins high dose group,cisplatin group,combined group: cisplatin combined with IP6 + Ins.3 The concentration of the drug used in the experiment and the preparation of the drug:blank control group: 0mmol/L,IP6+Ins low dose group: 1.5mmol/L,IP6+Ins middle dose group 3mmol/L,IP6+Ins high dose group 6mmol/L,cisplatin concentration 3ug/ml,combined group: concentration cisplatin: 3ug/m L+(IP6 + Ins): 3mmol/L.We dissolved the test drugs with 0.9% sodium chloride and stored them at 4?.4 The MTT assay was used to detect the proliferation of ovarian cancer SKOV3 cells in each experimental group after 24 h,48 h and 72 h,respectively.5 Western blot was used to detect the expression of Survivin in ovarian cancer SKOV3 cells.6 RT-PCR was used to detect the expression of JAK2 m RNA and STAT3 m RNA in ovarian cancer SKOV3 cells.7 statistical methods:In this study,SPSS13.0 statistical software was used to process the experimental data.The data were tested by normality test and variance homogeneity test.The t-test was used to compare the two samples.The single-factor analysis of variance was used to compare the number of samples.All of them were expressed as `x ± S,P <0.05 was statistically significant significance.Results:1 MTT colorimetric assay was used to detect the proliferation of ovarian cancer SKOV3 cells.At the same time and compared with the control group,the OD value was decreased,the difference was statistically significant(P <0.05).The proliferation of ovarian cancer SKOV3 cells was inhibited by the drug group.And this effect increased with the drug action time and IP6 + Ins concentration continuously;And there was a time-dose-effect relationship between the proliferation inhibition of IP6 + Ins groups.In this study,we compared the differences between the effects of the same combination of each time group,cisplatin group and IP6 + Ins high dose group on the proliferation of SKOV3 cells.Their OD values were ranked as the combination group<cisplatin group < IP6+Ins high dose group,the difference was statistically(P <0.05).In the same dose,the inhibition rate increased with the prolongation of the action time.The growth inhibition rates of the drugs after 72 h were: IP6+Ins low dose group: 10.86%,IP6+Ins middle dose group 22.94%,IP6+Ins high dose group: 33.64%,cisplatin group 44.95%,combined group 55.2%.2 The expression of Survivin in ovarian cancer SKOV3 cells was detected by Western blot:Survivin expression was present in all subgroups.It was highly expressed in the control group,However,it was lowly expressed in the experimental group,The expression of Survivin was determined as the following:the combined group< cisplatin group< IP6+Ins high dose group < IP6 + Ins middle dose group < IP6 + Ins low dose group <control group.3 RT-PCR was used to detect the expression of JAK2 m RNA and STAT3 m RNA in ovarian cancer SKOV3 cells: The JAK2 and STAT3 m RNA were expressed in all subgroups.They were highly expressed in the control group,However,they were was lowly expressed in the experimental groups.The expression of JAK2,STAT3 m RNA was as following: Combined group < cisplatin group < IP6+Ins high dose group < IP6+Ins middle dose group < IP6+Ins low dose group <control group,and the difference between the groups was statistically significant(P <0.05).Conclusions:IP6 + Ins can significantly inhibit the growth of ovarian cancer SKOV3 cells.Its possible mechanism is as following:The IP6+Ins reduces the expression of Survivin by downregulating the JAK2 / STAT3 signaling pathway promotes apoptosis.And IP6+Ins combined with cisplatin have a synergistic effect on this process.This study provides a sufficient theoretical basis for searching for the better treatment of ovarian cancer in clinical.