Association Of ADAMTS1 Gene Single Nucleotide Polymorphisms With Coronaryheart Disease In Chinese Han Population

Objective: To investigate the association of ADAMTS1 gene single nucleotide polymorphisms with atherosclerotic coronary heart disease(CHD).Methods: This study includes 315 CHD and 355 control who admitted to the First Affiliated Hospital of Chongqing Medical University.SNP rs428785 and SNP rs2738 were detected by polymerase chain reaction-single-strand conformation polymorphism(PCR-SSCP)and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP),then verified by gene sequencing techniques.Chi-square test was used to analyze the count data,Mann-Whitney U test and independent-samples T test were used to analyze the measurement data between the two groups.Results: The genotype frequencies of SNP rs428785 in CHD group and the control group respectively were CC(50.2% vs 38.9%),CG(40.3% vs 47.9%)and GG(9.5% vs 13.2%);there was a great statistically significance between the two groups(?2=8.974,P=0.011)as well as the frequency of G allele(29.7% vs 37.2%;?2=8.410,P=0.004).After logistic regression analysis,CG+GG genotype of SNPrs428785 may be associated with CHD(P=0.015,0R=1.528,95%CI=1.086 to 2.150).SNP rs2738 in CHD group and the control group were AA(74.3% vs 78.9%),AC(24.1% vs 18.9%)and CC(1.6% vs 2.3%)respectively,but there was no statistically significance between the two groups(?2=2.998,P=0.223),and there was also no significance in frequency of A allele(86.3% vs 88.3%;?2=1.164,P=0.281).Conclusion: SNP rs428785 but not SNP rs2738 polymorphisms of the ADAMTS1 gene may be associated with CHD.The G allele of the SNP rs428785 may be a genetic risk factor for CHD.