Ccl2 G-2578a Single Nucleotide Polymorphism And Blood Circulation Study On The Correlation Between Mcp-1 Levels And Coronary Heart Disease

Objective To detect the allele frequencies of the CCL2 G-2578 A polymorphism and whether plasma Monocyte Chemoattractant Protein-1(MCP-1) levels and the prevalent coronary atherosclerosis diseases are associated with CCL2 -2578G in Shandonger.Methods Polymerase chain reaction-restrict fragment length polymorphism (PCR-RFLP) was employed to detect the single nucleotide polymorphism(SNP) of CCL2 G-2578A in 110 cases and 79 gender- and age-matched healthy controls.Plasma MCP-1 levels of 49 randomly selected cases were measured by enzyme linked immunosorbent assay(ELISA),simultaneously highly-sensitive C-reactive protein (hsCRP) is tested by immune scattering rate turbidimetry.Results(1) The frequencies of A and G alleles in CCL2-2578 were 42.1%and 57.9%,respectively in Shandonger.(2) The percentage of AG+GG genotypes was significantly higher in the CAD group than the control group(87.1%vs.74.7%,OR=2.324, 95%CI 1.091-4.951,P=0.026).The association between the CCL2-2578G and CAD was no longer significant after adjustment for other well-established risk factors by binary logistic regression analysis(OR=2.370,95%CI 0.699-8.037,P=0.166).(3)The levels of plasma MCP-1 and hsCRP were not normal distribution,after log-transformed,the two variates were positive correlation by Pearson correlation analysis(r=0.535,P＜0.001), after adjustment for diabetes mellitus(DM),hypertension,IBM and genotype,the correlation was still remained(β=0.495,P=0.001) by multiple linear regresion analysis(Enter method);When the cases with measured MCP-1 were subdivided into AMI group(n=20) and non-AMI group(n=29),the AMI group had significant higher hsCRP(t=3.233,P=0.002),and marginal higher MCP-1(t=1.833,P=0.073).The levels of log-transformed MCP-1 in AA and AG+GG were not statistically significant, t=0.537,P=0.594.The levels of log-transformed MCP-1 was still not associated with genotypes(β=0.052,P=0.704),after adjustment for log-transformed hsCRP,DM, hypertension and IBM by multiple linear regression analysis.Conclusions(1) The frequencies of A and G alleles in CCL2 were 42.1%and 57.9%,respectively,in Shandonger.(2)The present study showed CCL2 -2578 G is a significant but not independent risk factor of CAD.(3)MCP-1 was positively related to hsCRP,MCP-1 had a higher level in AMI group than non-AMI group,but no statistical significance.MCP-1 was not associated with CCL2-2578G.