Effects Of FDI-6 On Malignant Behaviors In Human Laryngealcarcinoma Hep-2 Cell Line And The Underlying Mechanisms

Objective: To study the effects of new small molecular inhibitor FDI-6(Forkhead Domain Inhibitory-6,FDI-6)on the proliferation,apoptosis,migration and invasion ability in human laryngeal carcinoma Hep-2 cell line and the possible mechanisms.Methods: MTT assay was used to test the proliferation rate of Hep-2 cells before and 12,24 hours after treated with(5~80)?mol/L of FDI-6.Flow cytometry(FCM)and Transwell chamber assay were respectively carried out to detect apoptosis rate,cell migration and invasion ability in Hep-2 cells 24 h after treated by(10,20)?mol/L of FDI-6.Real-time quantitative PCR(qRT-PCR)and Western Blot were performed to determine the mRNA and protein level of FoxM1,Bcl-2 and Bax,respectively.Result: Cell proliferation rate was inhibited by FDI-6 in a dose-time dependent mannar(P<0.05).24 h after(10,20)?mol/LFDI-6 treatment,the apoptosis rate in Hep-2 cells was elevated(P<0.05)and the ability of cell migration and invasion were inhibited in a dose-time dependent mannar(P<0.05).The q RT-PCR showed that there was no significant change in FoxM1 mRNA expression with or without FDI-6 treatment(P>0.05).Western Blot showed that the total protein level of FoxM1 was no obvious changes(p>0.05),but Bcl-2 was down-regulated(p<0.05),Bax was up-regulated(p<0.05).However,nucleus FoxM1 protein level decreased along with the ascent of FDI-6 concentration.Conclusion: FDI-6 could induce cell apoptosis and inhibit cell proliferation,migration and invasion in Hep-2 cells.This may be the result of down-regulated FoxM1 in nucleus.