Plasma From Patients With Systemic Lupus Erythematosus Have Suppressive Effects On Mesenchymal Stem Cells

Objective : This research is to investigate whether plasma from patients with systemic lupus erythematosus(SLE)have the immunosuppressive effects on healthy human mesenchymal stem cells(MSCs).Methods:MSCs isolated and expanded from the bone marrow of healthy donors and then were co-cultured with B cells purified from the peripheral blood of SLE patients in the presence of fetal bovine serum(FBS)or pooled plasma from SLE patients(FBS was used as control group).The proliferation and maturation of B lymphocytes were analyzed by proliferation assay and flow cytometry(FCM).The experimental data were analysied with SPSS 12.0 student t test for statistical analysis,P < 0.05 means the difference between the two groups have statistical significance.Results:1? Isolation of MSCs from bone marrow and cell culture:MSCs were successfully cultured from human bone marrow.These cells were positive for CD44,CD73,CD90 and CD105,negative for CD34,CD45,CD14 and CD106,and could be induced to differentiate into osteocytes and adipocytes.2? Normal MSCs inhibited the proliferation of B lymphocytes from patients with SLE(Lipopolysaccharide were used to stimulate the proliferation of B cell).3? The pooled plasma from SLE patients significantly inhibited the suppressive effects of normal MSCs on B cell proliferation(Lipopolysaccharide were used to stimulate the proliferation of B cell).4? Normal MSCs suppressed the maturation of B lymphocytes from patients with SLE,which showed lowered expressions of CD27,CD38 and no change of CD19.5? The pooled plasma from SLE patients significantly suppressed the suppressive effects of normal MSCs on B cell maturation,which showed high level of CD27,CD38 and restrained expressions of CD19.Conclusion:Plasma from SLE patients negatively modulates the effects of normal MSCs in suppressing lupus B cell proliferation,maturation and change the proportion of B lymphocyte subsets.The pooled plasma from SLE patients inhibits the immunosuppressive effects of human MSCs,and negatively affect the therapeutic effect of MSCs transplantation for treatment of SLE.