| Poly β-hydroxybutyrate-co-hydroxyvalerate(PHBV)is a kind of biological material with excellent biocompatibility and biodegradability,and Polylactic acid(PLA)is also widely used in the medical field.PHBV/PLA material is blended by PHBV and PLA,and also performs excellent biocompatibility.This material has been made into filament and used in the development of medium-sized artificial vascular grafts.However,this kind of artificial vascular grafts made with PHBV/PLA are prone to collapse after implantation in vivo,due to its insufficient radical supporting force.This paper focuses on the structure design and the preparation process of the PHBV/PLA artificial blood vessel in order to improve its overall performance,especially the radial supporting force,facilitating the application of PHBV/PLA materials in the field of artificial blood vessels.Generally,the radial supporting force of the woven artificial blood vessels can be achieved through raw material preparation,and proper structure design and woven technology.Therefore,in order to improve the radial supporting force of an artificial blood vessel,the research was implemented from fiber material selection,structure desing and preparation process design to tests and analysis of related properties.Firstly,with testing and analyzing the performance of the PHBV/PLA multifilament,it was concluded that PHBV/PLA multifilament as the raw material was unable to provide sufficient radial supporting force for an artificial blood vessel.Therefore,in this research,the structure of the artificial blood vessel was studied and then the PET monofilament was introduced to improve the radial supporting performance.Additinally,in order to simulate the multi-layer structure of the biological artery,a weft double-based texture was designed with double-layer tube structure.The PHBV/PLA multifilament with excellent biocompatibility was used for the inner side of the artificial blood vessel,and good biocompatibility and biodegradability of PHBV/PLA could guarantee the biological properties of the artificial blood vessel.At the same time,the outer side was woven with PET monofilaments,providing good radial supporting force and elastic recovery rate for the artificial blood vessel.Therefore,the artificial blood vessel met the requirements of both biocompatibility and high supportability.Secondly,with references to the basic performance requirements of artificial blood vessels,sizing treatment was used to improve its weavability.In this paper,13 kinds of woven artificial vascular tubes with inner diameter of 8mm were successfully fabricated on the specially designed small loom through rational experimental design and weaving parameters,and then the artificial blood vessels were post-processed,including desizing,cleaning,heat setting and so on.The results showed that both warp and weft density were increased after these treatment,and yarn arrangement was more compact.Additionally,the geometry and biomechanical properties of the artificial blood vessels were tested,including wall thickness,inner diameter,water permeability,tensile strength,radial supporting force,elastic recovery rate and compliance.The radial supporting force was improved greatly,and the overall performance of this kind of artificial blood vessel was excellent.Finally,the biocompatibility of the artificial blood vessel was studied.Cytotoxicity of the artificial blood vessel was tested with the CCK-8 method,and the degree of cell activity was tested indirectly by measuring the absorbance value.The results showed that bioactivity of the PHBV/PLA artificial blood vessel with double-weft woven structures had reached to 80%,so its biocompatibility was good and the cytotoxicity was relatively low,which could be implanted in vivo.In this paper,the artificial blood vessels with high supportability and biocompatibility were developed by proper designs,providing the experimental and theoretical basis for the further study of the artificial blood vessels. |
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