Animal Model Establishment And Immunohistochemistry Of Self-made Modified Puncture Device

Objective:The animal model of rabbit VX2 bone metastases was established by experiment.The time of puncture biopsy was determined and the appropriate puncture time was determined.The effect of self-made puncture device was verified by PCNA immunohistochemistry.Methods:The animals used in the experiment were artificial breeding rabbits,the first part of the number of rabbits used in 25,the second part of 35,the experiment used a variety of equipment from the Chengde Medical College Hospital,the use of tumor cells and reagents purchased in the reagent company.1?the first part of the successful manufacture of rabbit VX2 tibial bone tumor model,by observing the imaging and pathological examination to determine the implantation of tumor tissue after the end of the second week,that is,on the 14 th day can be seen medullary tumor formation,and no violations of the cortex,The tumor tissue grew in the intramedullary muscle at1 to 2 weeks after implantation of the tumor cells in the left group.The fibrous tissue grew and the bone cortex was infused at the third week.The tumor tissue broke through the cortical bone around the 4th week.2?The second part of the experiment to verify the effect of homemade bone tumor puncture device,in the mature rabbit VX2 tibial bone tumor model and clear the medullary tumor formation but not yet break through the cortical phase of this time based on the PCNA immune Grouping technology to verify the effect of the device;Fifty rabbits were randomly divided into two groups: the left group and the right group,and the other 30 rabbits wererandomly divided into the control group and the control group.The left side of the group using self-made improved puncture needle in the cortical bone perforation after the closure,the right side of the application of traditional puncture needle puncture(at this time puncture needle in the cortex on the location of the implant should be implanted with the tumor the formation of the hole relative to avoid the formation of the mold when the impact of experimental results),After the completion of the two groups of puncture,the same layer of sterile dressing dressing(3 layers),the next day after the experiment observed two groups of experimental animal surgical site exudation,and then clear the two groups of experimental animals after surgery bleeding the two groups of experimental animals were sacrificed on the 10 th day after the completion of the puncture technique to obtain the soft tissue around the perforation of the puncture needle.The PCNA immunohistochemical technique was used to check the presence of tumor cells around the hole around the puncture needle.The effect of the device.Results:1?The first part of the successful manufacture of rabbit VX2 tibial bone tumor model,by observing imaging and pathological examination to determine the implantation of tumor tissue after the end of the second week,that is,the first 14 days can be seen medullary cavity tumor formation,and no violations of the cortex,the left the tumor tissue grew in the intramedullary tissue after 1 to 2 weeks of tumor implantation.The fibrous tissue grew and the bone cortex was infused at the third week.The tumor tissue broke through the cortical bone around the 4th week.2?The next day after the implementation of the puncture technique in 30 experimental animals,the left side of the surgical site dressings can be seen bloody exudate 1,the remaining dressing are dry and no bleeding;the right side of the surgical site of 25 visible a large number of bloody oozing,2 small bloody exudation,3 dressing dry and no exudation;the first 10 days of all experimental animals were sacrificed,to obtain the puncture needle formed by the surrounding soft tissue PCNA immunohistochemical treatment,the leftgroup that is self-made improved puncture needle there was no tumor cell invasion in the soft tissue around the puncture channel,and the tumor cells were invaded around the puncture hole.Conclusion:Has successfully established a rabbit VX2 bone tumor model,this model can be a good application in various types of bone tumors in animal experiments,the use of self-made puncture device puncture surgery,reducing the puncture after the implementation of the complications the incidence of self-made puncture device can be effectively applied to tumor biopsy,is a new type of effective devices and techniques.The device is characterized by a combination of various parts of the device,through the puncture needle through the cortical bone,the use of the device outside the casing to block the cortical bone and bone marrow cells,while the medullary cavity and the outside channel,in our access the need for adequate tissue,through the casing will be the key device that is closed into the puncture hole,and thus avoid the bone marrow cavity tumor cells through the puncture hole diffusion,and this method trauma than the traditional puncture needle no increase,but the prevention of complications after puncture has been significantly improved.The device can be in the pathological examination is not clear before,to minimize the proliferation of tumor tissue,and can reduce the puncture site bleeding and other complications,reduce the risk of hematoma formation,for clinical work has great significance.