The Study Of The Relationship Between The Expression Of The RhoA,ROCK1 And ROCK2 Proteins In Pancreatic Carcinoma And The Clinic Pathological Feature

Objective: Pancreatic cancer is a kind of digestion system malignant tumor with the highest malignancy,due to the dangerous state and the high mortality,it is one of the malignant tumors with the worst prognosis.In the recent years,the rate of surgical resection has been greatly improved,while the incidence of postoperative complications has been significantly reduced,however,the median survival time and five year survival rate get no obvious improvement.It is with significant meaning to get down to the study of the molecular mechanism of the growing and spreading,and to explore the new method of the treatment of the pancreatic carcinoma.Rho is a kind of small G proteins with GTPase activity,Rho kinase(ROCK)is the main effector molecules that can mediate the phosphorylation and dephosphorylation action and adjust many kinds of biological behavior after being activated by Rho kinase.Such as adjust the cell proliferation,the cytoskeleton activities,transformation,movement and adhere of cells,besides,it can also adjust the degradation of the ECM and angiogenesis,which is with very close relationship with the malignant tumor invasiveness and metastasis,which mechanism is very complicated.Recent research has found that the Rho/ROCK pathway often get abnormally activated in many kinds of malignant tumors such as liver cancer,breast cancer,lung cancer,stomach cancer and colon cancer,and it is highly correlated with the malignant tumor behavior.Some domestic researchers have conducted some research on the effect of the Rho family members on some malignant tumors such as esophagus cancer,prostate cancer and ovarian cancer,but no report on the Rho/ROCK pathway’s effect on the pancreatic carcinoma was found.In this study we applied the immunohistochemical method to detect the expression of RhoA,ROCK1 and ROCK2 protein in the pancreatic carcinoma tissue and normal pancreatic tissue,analyzed the relationship between the expression and the pancreatic carcinoma pathological feature,observed the relationship with the pancreatic biological behavior,combined the follow up data,preliminary discuss the meaning and value of the expression of the RhoA,ROCK1 and ROCK2 in pancreatic carcinoma,and provide the theoretical foundation for the pancreatic carcinoma treatment.Methods:1 Material 58 Pancreatic Ductal Adenocarcinoma paraffin specimens of the operations conducted between January 1st 2014 and December 31 st 2016 in Hebei medical university the 4th hospital were set to be experimental group.The selected patients underwent no chemotherapy,no radiotherapy and no immunotherapy,but all the cases were with full case history data and follow up data.16 cases of normal pancreas tissue obtained from the samples of pancreatic cystadenoma operations were set to be the control group.All the tissue samples were immobilized immediately after resected from the patients,and then paraffin embedded.The histology diagnosis were made by two experienced pathology doctors.2 Methods Immunohistochemical method was applied to detect the expression of the Rho,ROCK1 and ROCK2 proteins in the carcinoma tissue and normal tissue,SPSS17.0 software was applied to conduct the statistical analysis.Besides,the relationship between the expression of the Rho,ROCK1 and ROCK2 protein and the clinic and pathological features such as age,gender,tumor size,tumor location,lymph nodes metastasis situation,nerve affected situation,vessel affected situation,tumor differentiated degree and pathological grading was also statistically analyzed.The relationship between the expression of the Rho,ROCK1 and ROCK2 proteins and the prognosis of patients with pancreatic carcinoma was analyzed combined with the follow up data.Results:1 The expression of RhoA,ROCK1 and ROCK2 proteins in pancreatic carcinoma tissues are much higher than that in the normal pancreatic tissues;The positive rates of these proteins expression in carcinoma tissues are 77.6%,67.2%,74.1%;while in the normal pancreatic tissues are 18.8%,12.5%,12.5%.The difference is statistically significant(P<0.05).2 The difference between the positive expression of the RhoA,ROCK1,ROCK2 proteins and pathological grading,TNM stage,prognosis is statistically significant(P<0.05);While there is no statistically significant difference between the positive expression of the RhoA,ROCK1 and ROCK2 proteins and the patient age,gender,tumor location and vessel affected condition(P>0.05);The positive expression of the RhoA and ROCK2 proteins and the nerves invaded situation have statistically significant difference(P<0.05);There isn’t statistically significant difference between the positive expression of the ROCK1 and the nerves invaded situation(P>0.05).3 The half year survival rate and 1 year survival rate and 2 year survival rate of the RhoA,ROCK1 and ROCK2 protein in pancreatic carcinoma cells positive expression group was significantly lower than those of the negative group,and median survival time were significantly shorter than that of the negative group,the differences were statistically significant(P> 0.05).4 In the pancreatic carcinoma tissue,the expression of RhoA and ROCK1 are positively correlated with each other(r=0.479,P<0.05);The expression of RhoA and ROCK2 proteins are also positively correlated with each other(r=0.577,P<0.05);Besides,the expression of ROCK1 and ROCK2 proteins are also positively correlated with each other(r=0.347,P<0.05).Conclusions:1 The expression of RhoA,ROCK1 and ROCK2 proteins in pancreatic carcinoma tissues are much higher than that in the normal pancreatic tissues.2 The expression of RhoA,ROCK1 and ROCK2 proteins in the pancreatic carcinoma is closely correlated with the occurrence,development,invasion and metastasis.The positive expression of RhoA,ROCK1 and ROCK2 proteins often reminds a poor prognosis,so they can be regarded as indicators of the prognosis of pancreatic carcinoma.3 In the pancreatic carcinoma tissue,the expression of RhoA,ROCK1,ROCK2 are positively correlated with each other.Rho/ROCK signal transduction pathway may exists in pancreatic carcinoma tissue,which might become a new target in curing the pancreatic carcinoma.