The Expression Of LC3-Ⅱ,STS-1 And MyD88 In Peripheral Blood Lymphocytes Of Children With Systemic Juvenile Idiopathic Arthritis And The Correlation Study

Objective: To study the expression of LC3-Ⅱ in peripheral blood lymphocytes of children with systemic juvenile idiopathic arthritis(sJIA),we had a try to provide clues to further explore the role of autophagy in the development of sJIA.By studying the expression of MyD88 and STS-1,the MyD88-dependent Toll-like receptor signal pathway and STS-1 whether to participate in the occurrence of sJIA autophagy.And then to study whether there was a certain correlation,for the prevention and treatment of sJIA the incidence to provide new strategies and new targets,for clinical treatment to provide a new direction.Methods: The medical inpatient and outpatient children who have been diagnosed with sJIA and health children were selected from the Second Hospital of Hebei Medical University from May 2016 to January 2017.We employed "human peripheral blood lymphocyte separation medium" to separate lymphocytes.A comparison was made between healthy children who made health consultations in our hospital.We detected protein expression of lymphoid cells by Western blotting,and the data was measured by the statistical analysis.Immunofluorescence was used to detect the expression of LC3-Ⅱ in the cytoplasm of lymphocytes.Statistical processing methods: we used the variance analysis of the SPSS21.0 statistical software.Data obeyed the normal distribution was showed as mean ±standard deviation,and the comparison was the two sample t test.when data was non-normal distribution.We use median(interquartile range),and the comparison was the two sample application of nonparametric test.Linear correlation was used to analysis the relationship.P > 0.1 accorded with normal distribution,P < 0.05was considered statistically significant.P < 0.01 was considered significant difference.Results:1 In the systemic juvenile idiopathic arthritis group,the expression of STS-1 had a obvious statistical significance compared with the control group(P=0.000<0.05).The experimental group increased significantly because the experimental group and the control group were respectively(1.031±0.275)and(0.706±0.166).2 In the systemic juvenile idiopathic arthritis group,the expression of MyD88 had a obvious statistical significance compared with the control group(P=0.000<0.05).The experimental group increased significantly because the systemic juvenile idiopathic arthritis group and the control group were respectively(1.611±0.623)and(0.881±0.509).3 In the systemic juvenile idiopathic arthritis group,the expression of LC3-Ⅱhad a obvious statistical significance compared with the control group(P=0.004<0.05).The systemic juvenile idiopathic arthritis group increased significantly because the systemic juvenile idiopathic arthritis group and the control group were respectively(0.880±0.435)and(0.597±0.179).4 There was no correlation between the scores integral optical density of LC3-Ⅱ and STS-1 in peripheral blood lymphocytes of children with systemic idiopathic arthritis(sJIA),and there was no statistical significance by linear correlation analysis(Pearson Correlation=0.336,P=0.093>0.05).5 There was a positive correlation between the scores integral optical density of LC3-Ⅱ and MyD88 in peripheral blood lymphocytes of children with systemic idiopathic arthritis(sJIA),and there was statistical significance by linear correlation analysis(Pearson Correlation=0.478,P=0.013<0.05).6 There was a positive correlation between the scores integral optical density of STS-1 and MyD88 in peripheral blood lymphocytes of children with systemic idiopathic arthritis(sJIA),and there was statistical significance by linear correlation analysis(Pearson Correlation=0.817,P=0.000<0.05).Conclusions:1 The abnormal expression of STS-1 in peripheral blood lymphocytes of children with systemic juvenile idiopathic arthritis prompted that there may be differences in expression between normal children and children with sJIA.2 The high expression of LC3-Ⅱ in peripheral blood lymphocytes of children with systemic juvenile idiopathic arthritis proved the excessive expression of autophagy may participate in the onset of sJIA.3 The expression of MyD88 was abnormal in peripheral blood lymphocytes of children with systemic juvenile idiopathic arthritis,and there was linear correlation with the expression of LC3-Ⅱ.This phenomenon suggested that toll-like receptors signaling pathways were likely to involve in the occurrence of autophagy.4 There was no direct relation between the expression of LC3-Ⅱ and STS-1 in peripheral blood lymphocytes of children with systemic juvenile idiopathic arthritis.But we guessed that it was caused by small sample or individual differences,and there reported that STS-1 involved in the occurrence of autophagy.So we still though that STS-1 may activate certain signaling pathways to induce autophagy.