Intervention Of Brazilian Green Propolis In Peripheral Blood Inflammation Factors Of Patients With Mild Cognitive Impairment At Qinghai Tibet Plateau

Objective Discuss of Brazil propolis on inflammatory factor in patients with mild cognitive impairment in peripheral blood in Qinghai area regulation and intervention mechanism.Methods A randomized,double-blind,controlled trial methods of drugs,from an altitude of 2260 meters in December 2013 into the group of Han nationality in Xining area of urban residents in 60 MCI patients were randomly divided into treatment group(control group and Brazil oral placebo)30 cases,observation of Mini-Mental State Examination before treatment,and the curative effect of treatment in June 24 months.Enzyme-linked immunosorbent assay of the serum Interleukin-1 beta,Interleukin-6,Interleukin-10,Tumor necrosis factor-alpha,Interleukin-4,blood monocytes in Transforming growth factor.Reverse transcription PCR beta 1 level detection of peripheral IL-6,TGF-beta 1,Interferon-? level.Results 1.Compared to the control group,the two groups were compared to the age,gender,ethnic,cultural level of the mini-mental State Examination,and MMSE had no significant differences in all aspects(P>0.05).2.Compared with the control group for 24 months,the MMSE was increased in the treatment group and the difference was significant(P<0.01).Compared with control group treat different time:compared with the control group before treatment,the control group with placebo significantly lower MMSE score in June,24 months of the control group with placebo MMSE score reduced,significant difference(P<0.05,P<0.01);Compared with placebo in the control group,the placebo group received a significant reduction in MMSE in the period of 24 months,and the difference was significant(P<0.05).Treatment group compared to treat different time:prior to the treatment group and the treatment group than the Brazilian propolis six phases of the moon,24 months of treatment group take the Brazilian propolis MMSE score increased,the difference was significant(P<0.01);3.Using enzyme-linked immunosorbent assay methods:(1)set of comparison:the control group treatment 6 phases of the moon,compared with the treatment group treatment of inflammatory cytokines in peripheral blood of June I beta,IL-6,IL-TGF-beta 1 level differences were statistically significant(P<0.05),IL-10,the TNF alpha differences had no significance(P>0.05);The differences in the levels of inflammatory factors in the two groups of peripheral blood in 24 months,IL-1,IL-6,tgf-beta 1 and tnf-alpha were statistically significant(P<0.05).IL-10 no statistical significance(P>0.05);(2)compared group:(1)compared between control group:compared with the control group before the treatment and placebo six phases of the moon,and the control group with placebo serum inflammatory factors TGF-24 months of beta,IL-10 levels are increased,IL-6,IL-1 beta,reduced the level of TNF alpha,difference was statistically significant(P>0.05);(2)between the treatment group compared with the treatment group compared with before treatment,the treatment group take the Brazilian propolis inflammatory cytokines in serum IL-1 June beta level decreased,difference was significant(P>0.05);And treatment groups before treatment,the treatment group than the Brazilian propolis six phases of the moon,24 months of treatment group take the Brazilian propolis inflammatory cytokines in serum IL-6,IL-1 beta,TNF alpha were significantly reduced,TGF-beta levels increased,the difference had significance(P<0.05,P<0.01),there was no significant difference level of IL-10(P>0.05);4.Using reverse transcription polymerase chain reaction methods two groups of 24 months of treatment,in comparison with inflammatory factor levels in peripheral blood mononuclear cells,IL-6,IL-4,TGF-beta 1 differences were significant(P<0.05),INF-? there was no significance(P>0.05)difference gamma.Conclusion 1.The low oxygen environment and MCI have been closely associated with the occurrence,morbidity,and subsequent development of MCI,which can accelerate the disease progression of MCI patients and reduce the cognitive function of MCI patients;2.Brazilian propolis can improve the cognitive status of MCI patients in the plateau area,which could take up to two years;3.Brazilian propolis may in hypoxia environment by increasing nerve protective factor TGF beta 1 and IL-4 anti-inflammatory effects,and reduce inflammatory factor TNF alpha,beta,and the expression of IL-6 IL-1 to adjust,the serum levels of inflammatory markers and peripheral blood mononuclear cells are activated to restrain activity of microglia to improve cognitive function in patients with MCI in plateau area.