The Expression Of Cytokines In The Vitreous Of Proliferative Diabetic Retinopathy Patients With Different Clinical Feature

Purpose:To investigate the expression of vascular endothelial growth factor(VEGF),hypoxia-inducible factor-1?(HIF-1?),connective tissue growth factor(CTGF)and transforming growth factor ?2(TGF-?2)in the vitreous of PDR patients with different clinical feature.Methods:The vitreous samples were collected during the vitrectomy from 77 eyes of 68 patients with proliferative diabetic retinopathy(PDR)(experimental group),including 12 eyes with previous PRP treatment(PRP group)and 65 eyes without PRP treatment(NPRP group).The NPRP group was divided into tractional retinal detachment(TRD)group(15eyes),fibrosis membrane group(19 eyes),neovascularization membrane group(17 eyes)and vitreous hemorrhage group(14 eyes)further.16 eyes of 16 macular hole(MH)patients were considered as control group.The concentration of VEGF,HIF-1?,CTGF and TGF-?2 in vitreous was measured by enzyme-linked immunosorbent assay(ELISA).LSD-t test was used to analysis the differences between groups.Results:1.The concentration of VEGF,HIF-1?,CTGF and TGF-?2 in vitreous of experimental group was(3064.54±955.41)pg/ml,(0.672±0.204)ng/ml,(6.144±1.382)ng/ml and(3416.45±95.31)pg/ml respectively.In control group the concentration was(175.98±49.11)pg/ml,(0.331±0.028)ng/ml,(3.246±0.513)ng/ml and(713.64±182.45)pg/ml respectively,which was significantly lower than that of experimental group(P<0.05).2.The concentration of VEGF,HIF-1?,CTGF and TGF-?2 in vitreous of PRP group was(1035.76±438.54)pg/ml,(0.420±0.197)ng/ml,(4.109±0.774)ng/ml and(1049.09±158.53)pg/ml respectively.In NPRP group the concentration was(2157.59±1002.84)pg/ml,(0.578±0.203)ng/ml,(6.611±0.034)ng/ml and(2227.18±962.61)pg/ml respectively,which was significantly more than that of PRP group(P<0.05).3.VEGF: The concentration of VEGF in vitreous of TRD group,fibrosis membrane group,neovascularization membrane group and vitreous hemorrhage group was(2946.93±1097.16)pg/ml,(1915.74±985.04)pg/ml,(1897.80±842.71)pg/ml and(1573.62±606.33)pg/ml respectively,with the tendency of TRD group > fibrosis membrane group = neovascularization membrane group > vitreous hemorrhage group.4.HIF-1?: The concentration of HIF-1? in vitreous of TRD group,fibrosis membrane group,neovascularization membrane group and vitreous hemorrhage group was(0.637±0.195)ng/ml,(0.551±0.210)ng/ml,(0.549±0.187)ng/ml and(0.472±0.113)ng/ml respectively,with the tendency of TRD group > fibrosis membrane group =neovascularization membrane group > vitreous hemorrhage group.5.CTGF: The concentration of CTGF in vitreous of TRD group,fibrosis membrane group,neovascularization membrane group and vitreous hemorrhage group was(6.420±0.165)ng/ml,(5.767±0.124)ng/ml,(5.131±0.810)ng/ml and(4.507±0.712)ng/ml respectively,with the tendency of TRD group > fibrosis membrane group > neovascularization membrane group > vitreous hemorrhage group(P<0.05).6.TGF-?2 : The concentration of CTGF in vitreous of TRD group,fibrosis membrane group,neovascularization membrane group and vitreous hemorrhage group was(3105.29±931.71)pg/ml,(2658.55±873.01)pg/ml,(1918.26±114.58)pg/ml and(1327.18±602.63)pg/ml respectively,with the tendency of TRD group > fibrosis membrane group > neovascularization membrane group > vitreous hemorrhage group(P<0.05).Conclusions:The concentration of VEGF,HIF-1?,CTGF and TGF-?2 varies in eyes with the PDR progress,which showing different ocular fundus features.These four cytokines were associated with the severity of PDR condition.