The Preliminary Study Of Hsa_circ₀034495 And Hsa_circ₀016292 As A Biomarker Of Gastric Adenocarcinoma

Objective: To explore the expression level of hsa_circ₀016292 and hsa_circ₀034495 in gastric adenocarcinoma?GA?tissue and plasma,then analyze the relationship between their expression level and clinical pathological features.Finally,evaluate the value of hsa_circ₀016292 and hsa_circ₀034495 as a biomarker of gastric adenocarcinoma.Method:?1?Gene chips of high flux assay was used to detect the expression profiling of circRNA in GA tissues.Then,select the unreported and at least twofold statistically significant differential circRNAs as the targeted circRNA for this research.?2?The qRT-PCR technology were used to further verified the expression level of hsa_circ₀016292 and hsa_circ₀034495 in tissues?GA,para-carcinoma and chronic atrophic gastritis?and plasma?GA,chronic atrophic gastritis,colon cancer,rectal cancer and esophagus cancer?.Then,analyze the relationship between them and clinical pathological features,and evaluate their value as the biomarker of GA.Results:?1?Gene chips of high flux assay reveals that hsa_circ₀016292 and hsa_circ₀034495 are up-regulated in gastric adenocarcinoma compared to the paired adjacent non-neoplastic tissues..Verify the result of gene ships detect: The expression trends of hsa_circ₀034495 and hsa_circ₀016292 in GA tissues of sample enlargement was in accordance with gene ships of high flux assay.?2?The expression level of hsa_circ₀016292 and hsa_circ₀034495 in GA preoperative plasma were significantly higher than that in postoperative,colon cancer,rectal cancer,esophagus cancer and chronic atrophic gastritis?P <0.05?.Their level were negative relation with lymphatic metastasis,were independent of age,gender,invasion,differentiation,size,site,nerve invasion,vessel invasion,CEA and CA199?P > 0.05?.The level of hsa_circ₀016292 and hsa_circ₀034495 in GA plasma were positive relation,R value was 0.9107.Furthermore,the area under the ROC curve of hsa_circ₀034495 was 0.939,the sensitivity and specificity were 0.92 and 0.78;the area under the ROC curve of hsa_circ₀016292 was 0.940,the sensitivity and specificity were 0.91 and 0.85;when they were combined the area under the ROC curve was 0.944,the sensitivity and specificity were 0.84 and 0.92.Conclusion: The expression level of hsa_circ₀016292 and hsa_circ₀034495 in GA preoperative plasma were significantly higher than that in postoperative,colon cancer,rectal cancer,esophagus cancer and chronic atrophic gastritis,and their expression level were negative relation with lymphatic metastasis,but independent of others;Hsa_circ₀034495 and hsa_circ₀016292 had very high accuracy to diagnose GA,may be a perfect biomarker of GA.