Study On The EPR Effect Of Polymer Nano Micelles Invitro Renal Cancer Specimens

Object: 1.We build the perfusion model of human kidney tumorin vitro,and then use organ preservation solutiondissolving the polymer nano micelle which marked prolonium iodide to perfuse the kidney tumor circularly.2.Study the enhanced permeability retention effect(EPR effect)of polymer nano micelle in human kidney tumor and the quantitative relationship between them.Methods: 1.According to the cases of inclusion criteria choose to meet the requirements,and the patients signed the informed consent,preoperative and performer shows that experimental requirements,then catheterize the renal blood vessel immediately,and perfusethe kidney with organ preservation solution.2.Kidney tumor specimens after catheter was scanned by GE Discovery HD750 CT,and then connect the renal perfusion unit,will be dissolved with micellar organ preseration fluid filling kidney continuously,and scanned by GE Discovery HD750 CT GSI at 1.0h,2.0h,3.0h,4.0h,5.0h,6.0h;3.Continuous monitoring of infusion fluid PH,kidney produce the indicators such as urine,after the infusion,the kidney tumor specimens were fixed in 10% formalin,along with the rest of the specimens sent to the department.4.After the experiment we use GE ADW4.5 workstation to measure the polymer nano micelle and enrichment in kidney tumor samples quantity,draw diagrams and analysis results by SPSS19.0 statistical software.Results: 1.We build the perfusion model of renal tumor successfully,16 cases of kidney tumor specimens are completed smoothly perfusion,CT iodine base figureimages of micelle distribution were clear,during perfusion each kidney produce urine volume averaging were 40.05±5.22 ml,perfusion liquid PH stability between 7.35 to 7.45,perfusion pathology revealed after the ending of normal kidney tissue structure,no obvious edema,necrosis,and apoptosis ratio has no obvious change before and after the infusion(P > 0.05);2.The tumor area,normal renal cortex and medulla area of three different parts of the content of iodine,in front of the perfusion after the start of each time point and perfusion were compared,the results showed that the tumor area changes before and after the iodine content in perfusion was statistically significant(P = 0.00),at the beginning of the perfusion of iodine 1 h after the tumor area is obviously higher(1.79 ± 0.26)×100 ug/cm3,and evolve over timeshows higher iodine content and accumulation of the tumor area,and can maintain a certain time,increased to 3h as the highest peak,which was(2.19±0.36)×100 ug/cm3.The normal renal cortex and medulla before the start of the iodine content of each point in time than perfusion(P= 1.00)had no obvious change.3.Comparing three different particle size of polymer nano micelle filling kidney tumor specimens,we found that after infusion group size micelles of iodine in the tumor area,iodine base curve and peak time each are not identical: group A at the time of 4h iodine content is the highest,for(2.41±0.21)×100 ug/cm3,group B iodine content increased slowly,but iodine levels continue to rise for a long time,which means the micel would last for a long time;Iodine content starts up quickly in group C,at2 h goes to the top,(2.34±0.17)×100 ug/cm3,and decline slowly.In one word,EPR effect presents differently in different size of micellar,we did not find the obvious rule of them.Conclusion: 1.The perfusion model of of human kidney tumorin vitro is reliable,perfusion test do not affect the pathological results and kidney function,and it can obtain the real objective of the experimental results,and it provides a new methodfor secondary tumor specimens using research.2.By using the polymer nano micelle marked with prolonium iodide to conduct perfusion experiments,with the aid of precious stones energy spectrum CT iodine base figure technology,which can be accurately obtained polymer nano micelle in tumor tissues of enrichment,dynamic evolution law and micelle concentration of the quantitative parameters.3.EPR effect of polymer nano micelle does exist in vitro human renal cell carcinoma,and different size of polymer nano micelle in the amount of enrichment of tumor,and the trend evolution law have their own characteristics,which promptsthe complex circulation structure of tumor makes the EPR effect to be multiple.